User:Yeem/20.309/Mod 4 lab report

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(photobleach)
Current revision (11:42, 11 December 2007) (view source)
(+images)
 
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[[Image:yeem-intestinemucus.jpg|thumb|right|'''Figure 5.''' Blue channel of mouse intestine image, corresponding to mucus.]]
[[Image:yeem-intestinemucus.jpg|thumb|right|'''Figure 5.''' Blue channel of mouse intestine image, corresponding to mucus.]]
==Methods==
==Methods==
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Jaewon's microscope used a 5-watt 532nm pulse laser to provide excitation. The image dimensions were 256x256 pixels, saved as unsigned 16-bit integers in separate channels.
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Jaewon's microscope used a 5-watt 532nm pulse laser to provide excitation. The image dimensions were 256x256 pixels, saved as unsigned 16-bit integers in separate channels. The 3-D images were reconstructed using ImageJ's Volume Viewer plugin.
==Discussion==
==Discussion==
===Beads===
===Beads===

Current revision

Figure 1. 1μm beads with z-ratio of 0.5
Figure 1. 1μm beads with z-ratio of 0.5
Figure 2. Mouse neurons imaged with z-ratio of 3.0
Figure 2. Mouse neurons imaged with z-ratio of 3.0
Figure 3. Red channel of mouse intestine image, corresponding to actin filaments.
Figure 3. Red channel of mouse intestine image, corresponding to actin filaments.
Figure 4. Green channel of mouse intestine image, corresponding to cell nuclei.
Figure 4. Green channel of mouse intestine image, corresponding to cell nuclei.
Figure 5. Blue channel of mouse intestine image, corresponding to mucus.
Figure 5. Blue channel of mouse intestine image, corresponding to mucus.

Contents

Methods

Jaewon's microscope used a 5-watt 532nm pulse laser to provide excitation. The image dimensions were 256x256 pixels, saved as unsigned 16-bit integers in separate channels. The 3-D images were reconstructed using ImageJ's Volume Viewer plugin.

Discussion

Beads

The beads appeared elongated and slightly deformed, likely an artifact of the field of excitation.

Mouse intestine

A square area in the upper right (x-y plane) was photobleached by accident.

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