User:Yuri Hanada

Contact Info

• Yuri Hanada and Sohyun Park
• Massachusetts Institute of Technology
• Email us through OpenWetWare

Module 3

Project References

<biblio>

1. Paper1 pmid=18352826

// In this study, researchers incorporated demineralized bone particles (DBPs) into poly-lactide-co-glycolic acid (PLGA) scaffolds to study the anti-inflammatory effects of DBPs. While PLGA scaffolds are biocompatible and approved by the U.S. FDA, their acidic degradation products can decrease the pH of the surrounding tissue, which results in local inflammation and subsequent poor tissue development. In previous studies, DBPs have been shown to induce osteogenesis without fibrotic capsule formation and enhance PLGA hydrophilicity, preventing adverse cellular response. Scaffolds were fabricated and impregnated with DBPs via a solvent casting-salt leaching method, and then NIH/3T3 fibroblasts were seeded onto the scaffolds. A DBP-impregnanted PLGA film was also constructed to evaluate the inflammatory response of HL-60 cells in vitro via RT-PCR analysis of TNF-α and IL-1β mRNA expression. PLGA films with varying percentages of DBPs were additionally implanted into rats. In vitro, both TNF-α and IL-1β mRNA were found to be more highly expressed on PLGA film versus DBP-PLGA films. In vivo, histology indicated that the inflammatory reaction surrounding the PLGA film was progressively diminished as the DBP content increased

1. Paper2 pmid=18427294

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