User:Yvonne Lipsewers page: Difference between revisions
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I am a PhD student in the Department of Marine Organic Biogeochemistry at the Royal Netherlands Institute for Sea Research (NIOZ). | I am a PhD student in the Department of Marine Organic Biogeochemistry at the Royal Netherlands Institute for Sea Research (NIOZ). | ||
= | RESEARCH INTERESTS | ||
*Marine Microbiology and Geomicrobiology | |||
*Relevance of chemolithoautotrophic microorganisms for carbon fixation in marine sediments | |||
*Biomarker analysis (DNA/RNA & Lipids) | |||
=PhD project= | |||
Tracing chemoautotrophic microbes in present and past sedimentary environments | |||
In this project we will apply a combined approach based on the analysis of signature lipid biomarkers and functional gene analysis to study the diversity and activity of chemoautotrophic microorganisms in marine sediments. We will identify the carbon fixation pathways used by chemolithoautotrophs by quantifying the isotopic fractionation patterns of lipids and biomass in pure cultures and sediment incubations by stable carbon isotope analyses and by targeting genes of carbon fixation and re-oxidation pathways. The results from pure culture and sediment incubation studies will be used to examine the occurrence and role of chemolithoautotrophic microorganisms in ancient sediments by focusing on specific biomarker lipid molecules preserved in the geological record. | |||
People involved: Y. Lipsewers (Ph.D student), Dr. L. Villanueva, | |||
Prof. Dr. S. Schouten, Prof. J.S. Sinninghe Damsté (promotor) | |||
Funding: Darwin Center for Biogeosciences | |||
Duration: 2011-2014 |
Revision as of 06:23, 16 December 2014
Yvonne Lipsewers
I am a PhD student in the Department of Marine Organic Biogeochemistry at the Royal Netherlands Institute for Sea Research (NIOZ).
RESEARCH INTERESTS
- Marine Microbiology and Geomicrobiology
- Relevance of chemolithoautotrophic microorganisms for carbon fixation in marine sediments
- Biomarker analysis (DNA/RNA & Lipids)
PhD project
Tracing chemoautotrophic microbes in present and past sedimentary environments
In this project we will apply a combined approach based on the analysis of signature lipid biomarkers and functional gene analysis to study the diversity and activity of chemoautotrophic microorganisms in marine sediments. We will identify the carbon fixation pathways used by chemolithoautotrophs by quantifying the isotopic fractionation patterns of lipids and biomass in pure cultures and sediment incubations by stable carbon isotope analyses and by targeting genes of carbon fixation and re-oxidation pathways. The results from pure culture and sediment incubation studies will be used to examine the occurrence and role of chemolithoautotrophic microorganisms in ancient sediments by focusing on specific biomarker lipid molecules preserved in the geological record.
People involved: Y. Lipsewers (Ph.D student), Dr. L. Villanueva, Prof. Dr. S. Schouten, Prof. J.S. Sinninghe Damsté (promotor) Funding: Darwin Center for Biogeosciences Duration: 2011-2014