User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/09/24: Difference between revisions
(→Notes) |
(→Notes) |
||
Line 35: | Line 35: | ||
Thank you to Carlina for making more Hemoglobin 5mg/ml for us. | Thank you to Carlina for making more Hemoglobin 5mg/ml for us. | ||
Today we were able to complete the UV Vis for Pepsin at 0.5 hrs and 1 hr and the pepstatin for 1 hour. We put all of our SDS page samples in the fridge, and tomorrow we will run a gel with those samples. We still have to complete all the UV Vis tomorrow for the remaining samples of pepstatin and pepsin. The absorption graph for the pepsin and pepstatin will be shown on the following days lab. | |||
Revision as of 13:50, 24 September 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo observe the catalytic activity of pepsin (in cleaving peptide bonds in hemoglobin) in the presence and absence of pepstatin. This data will be compared with data we take in a later lab on pepsin-AuNPs. DescriptionThe experimental details (pepsin cleavage of hemoglobin) are similar to this reference. We will allow the pepsin cleavage of hemoglobin to proceed for two hours with aliquots taken and analyzed every 1/2 hour. We will also run parallel reactions with different concentrations of pepstatin (pepsin inhibitor) present. We will save our aliquots for analysis tomorrow using SDS-PAGE.
Data
NotesThank you to Carlina for making more Hemoglobin 5mg/ml for us. Today we were able to complete the UV Vis for Pepsin at 0.5 hrs and 1 hr and the pepstatin for 1 hour. We put all of our SDS page samples in the fridge, and tomorrow we will run a gel with those samples. We still have to complete all the UV Vis tomorrow for the remaining samples of pepstatin and pepsin. The absorption graph for the pepsin and pepstatin will be shown on the following days lab.
|