User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/09/25: Difference between revisions
From OpenWetWare
(→Notes) |
|||
Line 35: | Line 35: | ||
==Notes== | ==Notes== | ||
Table 1: Samples in Welss | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Well Numbers''' | |||
| align="center" style="background:#f0f0f0;"|'''Sample''' | |||
|- | |||
| 1||Hemoglobin | |||
|- | |||
| 2||Pepsin | |||
|- | |||
| 3||Pepstatin | |||
|- | |||
| 4||0.5hrs Pepsin | |||
|- | |||
| 5||0.5 hrs Pepstatin | |||
|- | |||
| 6||1 hr Pepsin | |||
|- | |||
| 7||1 hr Pepstatin | |||
|- | |||
| 8||1.5hrs Pepsin | |||
|- | |||
| 9||1.5 hrs Pepstatin | |||
|- | |||
| 10||2hrs Pepsin | |||
|- | |||
| 11||2hrs Pepstatin | |||
|- | |||
| 12||Hemoglobin | |||
|- | |||
| | |||
|} | |||
__NOTOC__ | __NOTOC__ |
Revision as of 10:06, 25 September 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo run SDS-PAGE to observe amount of hemoglobin remaining after yesterday's digestion. This should be a complimentary measurement to our UV-Vis analysis from yesterday. DescriptionWe will be using a Bio-Rad Mini Protean system with pre-cast Mini Protean TGX gels. The manual for this system can be found here We will be running SDS-PAGE followed by gel development with Coomassie Blue staining. Below is a short description of how we will proceed. Please refer to the manual for more detailed instructions
|
Notes
Table 1: Samples in Welss
Well Numbers | Sample |
1 | Hemoglobin |
2 | Pepsin |
3 | Pepstatin |
4 | 0.5hrs Pepsin |
5 | 0.5 hrs Pepstatin |
6 | 1 hr Pepsin |
7 | 1 hr Pepstatin |
8 | 1.5hrs Pepsin |
9 | 1.5 hrs Pepstatin |
10 | 2hrs Pepsin |
11 | 2hrs Pepstatin |
12 | Hemoglobin |