User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/01: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Notes== | ==Notes== | ||
Making 1/9 dilution of luminol | |||
1/100 HRP | |||
Figure 1. Corrected Absorbance Spectra of preliminary kinetics | |||
<br> | |||
[[Image:HRP diluteluminol h2o2 zem.png|750px]] | |||
<br> | |||
Figure 2. Absorbance spectra of the oxidation of luminol by HRP focusing on the 350nm region | |||
[[Image:Kineticsmonitoringzem10012013.png|750px]] | |||
<br> | |||
*The wavelength maximum occurs at 348.99nm and experiences a growth in signal. This indicates the production of 3-aminophthalic acid. | |||
<br> | |||
Figure 3. Absorption spectra of the production of 3-aminopthalate by the oxidaiton of luminol by HRP focusing on the 420nm region''' | |||
[[Image:Kinematicsincreaseprdtzem10012013.png|750px]] | |||
<br> | |||
*The wavelength maximum occurs at 415.42nm. During the course of the ten minutes, a signal reduction occurred, indicating the oxidation of luminol | |||
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Latest revision as of 23:24, 26 September 2017
Project name | Main project page Previous entry Next entry |
ObjectiveTo monitor the kinetics and yield of the horseradish peroxidase-catalyzed oxidation of luminol. These experiments will be compared to future experiments with HRP-functionalized nanoparticles. These experiments are also meant to introduce researchers to stopped-flow techniques and rapid data collection. DescriptionThree sets of measurements will be performed today.
DataStock Solution
NotesMaking 1/9 dilution of luminol 1/100 HRP Figure 1. Corrected Absorbance Spectra of preliminary kinetics
Figure 2. Absorbance spectra of the oxidation of luminol by HRP focusing on the 350nm region
Figure 3. Absorption spectra of the production of 3-aminopthalate by the oxidaiton of luminol by HRP focusing on the 420nm region
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