User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/01: Difference between revisions
From OpenWetWare
(→Notes) |
(→Notes) |
||
Line 65: | Line 65: | ||
[[Image:Kinematicsincreaseprdtzem10012013.png|750px]] | [[Image:Kinematicsincreaseprdtzem10012013.png|750px]] | ||
<br> | <br> | ||
*The wavelength maximum occurs at 415.42nm. During the course of the ten minutes, a signal reduction occurred, indicating the oxidation of luminol | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 16:31, 1 December 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo monitor the kinetics and yield of the horseradish peroxidase-catalyzed oxidation of luminol. These experiments will be compared to future experiments with HRP-functionalized nanoparticles. These experiments are also meant to introduce researchers to stopped-flow techniques and rapid data collection. DescriptionThree sets of measurements will be performed today.
DataStock Solution
NotesMaking 1/9 dilution of luminol 1/100 HRP Figure 1. Corrected Absorbance Spectra of preliminary kinetics
Figure 2. Absorbance spectra of the oxidation of luminol by HRP focusing on the 350nm region
Figure 3. Absorption spectra of the production of 3-aminopthalate by the oxidaiton of luminol by HRP focusing on the 420nm region
|