User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/15: Difference between revisions

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==Entry title==
* Insert content here...


==Description==
# Make 4 solutions of HRP-AuNPs
## Au:HRP ratio - 30:1 HRP-Au (final concentration of Au - 0.25mM; total volume - 10mL)
## Au:HRP ratio - 60:1 HRP-Au (final concentration of Au - 0.25mM; total volume - 10mL)
## Au:HRP ratio - 90:1 HRP-Au (final concentration of Au - 0.25mM; total volume - 10mL)
## Au:HRP ratio - 120:1 HRP-Au (final concentration of Au - 0.25mM; total volume - 10mL)
==Data==
Stock solutions
# Gold
## 25.0mg HAuCl<sub>4</sub>·H<sub>2</sub>O in 25.0mL water --> 2.94mM
# HRP
## 17.9mg HRP in 25.0mL water --> 16.27μM
==Notes==


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Revision as of 11:18, 15 October 2013

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Description

  1. Make 4 solutions of HRP-AuNPs
    1. Au:HRP ratio - 30:1 HRP-Au (final concentration of Au - 0.25mM; total volume - 10mL)
    2. Au:HRP ratio - 60:1 HRP-Au (final concentration of Au - 0.25mM; total volume - 10mL)
    3. Au:HRP ratio - 90:1 HRP-Au (final concentration of Au - 0.25mM; total volume - 10mL)
    4. Au:HRP ratio - 120:1 HRP-Au (final concentration of Au - 0.25mM; total volume - 10mL)

Data

Stock solutions

  1. Gold
    1. 25.0mg HAuCl4·H2O in 25.0mL water --> 2.94mM
  2. HRP
    1. 17.9mg HRP in 25.0mL water --> 16.27μM

Notes