User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/16

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Objective

We are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol

Description

  1. Add experimental record here. Include what, how, and why...

Data

Stock Solution

  1. Buffer
    1. 0.6140g Tris in 1L, pH set to 8 with HCl ---> 5.1mM
  2. Luminol
    1. Dissolve 12.9mg luminol in 300uL of DMSO
    2. Add to 50mL buffer ---> 1.46mM
  3. H2O2
    1. 312uL 30% H2O2 into 100mL buffer ---> Should be 45mM
    2. Check absorption at 250 source (ε(250) = 16.69 M-1cm-1). A = 0.7392. [H2O2] = 44.29mM

Notes

- We measured the absorbance of the 1/20 luminol to see if it still absorbed. We found the absorbance peak to be less than 1.00, specifically around 0.300

Graph showing the absorbance spectra for citrate AuNP and HRP AuNP


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