User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/30

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==Entry title==
==Entry title==
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Today we created new nanoparticles with myoglobin and lysozyme in order to determine the optimal concentration for fiber formation.
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==Notes==
==Notes==

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Entry title

Today we created new nanoparticles with myoglobin and lysozyme in order to determine the optimal concentration for fiber formation.

Notes

Today we are making HRP and myoglobin and pepsin nanoparticles

Table 1. Preparation of Myoglobin-AuNP solutions

Ratio Myoglobin Amount of gold added(mL) Concentration Myoglobin(M) Volume Myoglobin(mL) Volume Water Myoglobin(mL)
50.4942645580.0000522.505735442
100.4942645580.00002513.505735442
150.4942645581.66667E-050.6666666673.839068775
200.4942645580.00001250.54.005735442
250.4942645580.000010.44.105735442
300.4942645588.33333E-060.3333333334.172402108
350.4942645587.14286E-060.2857142864.220021156
400.4942645580.000006250.254.255735442
450.4942645585.55556E-060.2222222224.28351322
500.4942645580.0000050.24.305735442


Table 2. Preparation of all Lysozyme-AuNP solutions

Ratio Lysozyme Amount gold added (mL) Concentration Lysozyme(M) Volume Lysozyme (mL) Volume Water Lysozyme (mL)
300.4942645588.33333E-061.6111486492.894586793
500.4942645580.0000050.9666891893.539046253
700.4942645583.57143E-060.6904922783.815243164
900.4942645582.77778E-060.537049553.968685892
1100.4942645582.27273E-060.4394041774.066331265
1300.4942645581.92308E-060.3718035344.133931907
1500.4942645581.66667E-060.322229734.183505712
1700.4942645581.47059E-060.284320354.221415092
1900.4942645581.31579E-060.2543918924.25134355
2100.4942645581.19048E-060.2301640934.275571349


Table 3. Stock Solution concentrations

Concentration of Myoglobin (M) Concentration Gold(M) Concentration Lysozyme (M)
0.0001250.002529012.58615E-05


Table 4. Preparation of Lysozyme Stock solution

Molecular Weight(g/mol) 14307
mass lysozyme(g)0.0037
volume solution(mL)10
Concentration Lysozyme (M)2.58615E-05


Table 5. Preparation of Myoglobin Stock solution

Molecular weight (g/mol) 17600
mass myoglobin (g)0.022
volume solution (mL)0.01
Concentration (M)0.000125



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