User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/11/06: Difference between revisions

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==Entry title==
==What we did today==
 
Today we analyzed our lysozyme and myoglobin nano particles using UV-Vis. We then created new hemoglobin and lysozyme nanoparticles in different ratios than before.


==Notes==
==Notes==
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[[Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions.png]]
[[Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions.png]]
<br>
<br>
*only the 30 to 1 exhibited an absorbance peak where it was expected






Figure 2. Corrected Absorbance Spectra of Myoglobin Au-NP solutions
<br>
[[Image:Corrected Absorbance spectra of Myoglobin Au-NP solution .png]]
<br>
* 15 to 1 is the only ratio that produced 730nm absorbance peak




GOLD NANO SETUP!!!


 
Table 1. Preparation of Hemoglobin Stock solution
 
<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Molecular weight (g/mol)'''
| align="center" style="background:#f0f0f0;"|'''64500'''
|-
| mass myoglobin (g)||0.0106
|-
| volume solution (L)||0.01
|-
| Concentration (M)||1.64341E-05
|}
<br>
Table 2. Preparation of Gold stock solution
<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Molecular weight Au (g/mol)'''
| align="center" style="background:#f0f0f0;"|'''393.83'''
|-
| mass Au(g)||0.032
|-
| volume stock(L)||0.025
|-
| Concentration (M)||0.003250133
|}
<br>
Table 3. Preparation of Lysozyme stock solution
<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Molecular weight (g/mol)'''
| align="center" style="background:#f0f0f0;"|'''14307'''
|-
| mass lysozyme (g)||0.0717
|-
| volume solution (L)||0.05
|-
| Concentration (M)||0.00010028
|}
*Note: This stock solution was prepared by [[User:James C. Schwabacher/Notebook/CHEM-571/2013/11/06|James Schwabacher]]
<br>
Table 4. Preparation of Hemoglobin-AuNP solutions
<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Ratio'''
| align="center" style="background:#f0f0f0;"|'''Amount of gold added'''
| align="center" style="background:#f0f0f0;"|'''concentration hemoglobin (M)'''
| align="center" style="background:#f0f0f0;"|'''volume Hemoglobin (mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume Water (mL)'''
|-
| 30||0.384599609||8.33333E-06||2.535377358||2.080023032
|-
| 50||0.384599609||0.000005||1.521226415||3.094173976
|-
| 70||0.384599609||3.57143E-06||1.086590296||3.528810094
|-
| 90||0.384599609||2.77778E-06||0.845125786||3.770274604
|-
| 110||0.384599609||2.27273E-06||0.691466552||3.923933838
|-
| 130||0.384599609||1.92308E-06||0.585087083||4.030313308
|-
| 150||0.384599609||1.66667E-06||0.507075472||4.108324919
|-
| 170||0.384599609||1.47059E-06||0.447419534||4.167980857
|-
| 190||0.384599609||1.31579E-06||0.400322741||4.21507765
|-
| 210||0.384599609||1.19048E-06||0.362196765||4.253203625
|}
<br>
Table 5. Preparation of Lysozyme Au-NP solutions
<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Ratio'''
| align="center" style="background:#f0f0f0;"|'''Amount gold added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Concentration Lysozyme (M)'''
| align="center" style="background:#f0f0f0;"|'''Volume lysozyme (mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume water (mL)'''
|-
| 5||0.384599609||0.00005||2.493019545||2.122380845
|-
| 10||0.384599609||0.000025||1.246509773||3.368890618
|-
| 15||0.384599609||1.66667E-05||0.831006515||3.784393876
|-
| 20||0.384599609||0.0000125||0.623254886||3.992145504
|-
| 25||0.384599609||0.00001||0.498603909||4.116796482
|-
| 30||0.384599609||8.33333E-06||0.415503258||4.199897133
|-
| 35||0.384599609||7.14286E-06||0.356145649||4.259254741
|-
| 40||0.384599609||0.00000625||0.311627443||4.303772947
|-
| 45||0.384599609||5.55556E-06||0.277002172||4.338398219
|-
| 50||0.384599609||0.000005||0.249301955||4.366098436
|}




Revision as of 16:19, 1 December 2013

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What we did today

Today we analyzed our lysozyme and myoglobin nano particles using UV-Vis. We then created new hemoglobin and lysozyme nanoparticles in different ratios than before.

Notes

Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions


  • only the 30 to 1 exhibited an absorbance peak where it was expected


Figure 2. Corrected Absorbance Spectra of Myoglobin Au-NP solutions

  • 15 to 1 is the only ratio that produced 730nm absorbance peak


GOLD NANO SETUP!!!

Table 1. Preparation of Hemoglobin Stock solution

Molecular weight (g/mol) 64500
mass myoglobin (g) 0.0106
volume solution (L) 0.01
Concentration (M) 1.64341E-05


Table 2. Preparation of Gold stock solution

Molecular weight Au (g/mol) 393.83
mass Au(g) 0.032
volume stock(L) 0.025
Concentration (M) 0.003250133


Table 3. Preparation of Lysozyme stock solution

Molecular weight (g/mol) 14307
mass lysozyme (g) 0.0717
volume solution (L) 0.05
Concentration (M) 0.00010028


Table 4. Preparation of Hemoglobin-AuNP solutions

Ratio Amount of gold added concentration hemoglobin (M) volume Hemoglobin (mL) Volume Water (mL)
30 0.384599609 8.33333E-06 2.535377358 2.080023032
50 0.384599609 0.000005 1.521226415 3.094173976
70 0.384599609 3.57143E-06 1.086590296 3.528810094
90 0.384599609 2.77778E-06 0.845125786 3.770274604
110 0.384599609 2.27273E-06 0.691466552 3.923933838
130 0.384599609 1.92308E-06 0.585087083 4.030313308
150 0.384599609 1.66667E-06 0.507075472 4.108324919
170 0.384599609 1.47059E-06 0.447419534 4.167980857
190 0.384599609 1.31579E-06 0.400322741 4.21507765
210 0.384599609 1.19048E-06 0.362196765 4.253203625


Table 5. Preparation of Lysozyme Au-NP solutions

Ratio Amount gold added (mL) Concentration Lysozyme (M) Volume lysozyme (mL) Volume water (mL)
5 0.384599609 0.00005 2.493019545 2.122380845
10 0.384599609 0.000025 1.246509773 3.368890618
15 0.384599609 1.66667E-05 0.831006515 3.784393876
20 0.384599609 0.0000125 0.623254886 3.992145504
25 0.384599609 0.00001 0.498603909 4.116796482
30 0.384599609 8.33333E-06 0.415503258 4.199897133
35 0.384599609 7.14286E-06 0.356145649 4.259254741
40 0.384599609 0.00000625 0.311627443 4.303772947
45 0.384599609 5.55556E-06 0.277002172 4.338398219
50 0.384599609 0.000005 0.249301955 4.366098436



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