User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/11/06

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==Entry title==
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==What we did today==
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Today we analyzed our lysozyme and myoglobin nano particles using UV-Vis. We then created new hemoglobin and lysozyme nanoparticles in different ratios than before.
==Notes==
==Notes==

Revision as of 19:18, 1 December 2013

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What we did today

Today we analyzed our lysozyme and myoglobin nano particles using UV-Vis. We then created new hemoglobin and lysozyme nanoparticles in different ratios than before.

Notes

Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions

Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions.png

  • only the 30 to 1 exhibited an absorbance peak where it was expected


Figure 2. Corrected Absorbance Spectra of Myoglobin Au-NP solutions
Image:Corrected Absorbance spectra of Myoglobin Au-NP solution .png

  • 15 to 1 is the only ratio that produced 730nm absorbance peak


GOLD NANO SETUP!!!

Table 1. Preparation of Hemoglobin Stock solution

Molecular weight (g/mol) 64500
mass myoglobin (g)0.0106
volume solution (L)0.01
Concentration (M)1.64341E-05


Table 2. Preparation of Gold stock solution

Molecular weight Au (g/mol) 393.83
mass Au(g)0.032
volume stock(L)0.025
Concentration (M)0.003250133


Table 3. Preparation of Lysozyme stock solution

Molecular weight (g/mol) 14307
mass lysozyme (g)0.0717
volume solution (L)0.05
Concentration (M)0.00010028


Table 4. Preparation of Hemoglobin-AuNP solutions

Ratio Amount of gold added concentration hemoglobin (M) volume Hemoglobin (mL) Volume Water (mL)
300.3845996098.33333E-062.5353773582.080023032
500.3845996090.0000051.5212264153.094173976
700.3845996093.57143E-061.0865902963.528810094
900.3845996092.77778E-060.8451257863.770274604
1100.3845996092.27273E-060.6914665523.923933838
1300.3845996091.92308E-060.5850870834.030313308
1500.3845996091.66667E-060.5070754724.108324919
1700.3845996091.47059E-060.4474195344.167980857
1900.3845996091.31579E-060.4003227414.21507765
2100.3845996091.19048E-060.3621967654.253203625


Table 5. Preparation of Lysozyme Au-NP solutions

Ratio Amount gold added (mL) Concentration Lysozyme (M) Volume lysozyme (mL) Volume water (mL)
50.3845996090.000052.4930195452.122380845
100.3845996090.0000251.2465097733.368890618
150.3845996091.66667E-050.8310065153.784393876
200.3845996090.00001250.6232548863.992145504
250.3845996090.000010.4986039094.116796482
300.3845996098.33333E-060.4155032584.199897133
350.3845996097.14286E-060.3561456494.259254741
400.3845996090.000006250.3116274434.303772947
450.3845996095.55556E-060.2770021724.338398219
500.3845996090.0000050.2493019554.366098436



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