Entry title
Notes
Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions
- only the 30 to 1 exhibited an absorbance peak where it was expected
Figure 2. Corrected Absorbance Spectra of Myoglobin Au-NP solutions
- 15 to 1 is the only ratio that produced 730nm absorbance peak
GOLD NANO SETUP!!!
Table 1. Preparation of Hemoglobin Stock solution
Molecular weight (g/mol)
|
64500
|
mass myoglobin (g) |
0.0106
|
volume solution (L) |
0.01
|
Concentration (M) |
1.64341E-05
|
Table 2. Preparation of Gold stock solution
Molecular weight Au (g/mol)
|
393.83
|
mass Au(g) |
0.032
|
volume stock(L) |
0.025
|
Concentration (M) |
0.003250133
|
Table 3. Preparation of Lysozyme stock solution
Molecular weight (g/mol)
|
14307
|
mass lysozyme (g) |
0.0717
|
volume solution (L) |
0.05
|
Concentration (M) |
0.00010028
|
Table 4. Preparation of Hemoglobin-AuNP solutions
Ratio
|
Amount of gold added
|
concentration hemoglobin (M)
|
volume Hemoglobin (mL)
|
Volume Water (mL)
|
30 |
0.384599609 |
8.33333E-06 |
2.535377358 |
2.080023032
|
50 |
0.384599609 |
0.000005 |
1.521226415 |
3.094173976
|
70 |
0.384599609 |
3.57143E-06 |
1.086590296 |
3.528810094
|
90 |
0.384599609 |
2.77778E-06 |
0.845125786 |
3.770274604
|
110 |
0.384599609 |
2.27273E-06 |
0.691466552 |
3.923933838
|
130 |
0.384599609 |
1.92308E-06 |
0.585087083 |
4.030313308
|
150 |
0.384599609 |
1.66667E-06 |
0.507075472 |
4.108324919
|
170 |
0.384599609 |
1.47059E-06 |
0.447419534 |
4.167980857
|
190 |
0.384599609 |
1.31579E-06 |
0.400322741 |
4.21507765
|
210 |
0.384599609 |
1.19048E-06 |
0.362196765 |
4.253203625
|
Table 5. Preparation of Lysozyme Au-NP solutions
Ratio
|
Amount gold added (mL)
|
Concentration Lysozyme (M)
|
Volume lysozyme (mL)
|
Volume water (mL)
|
5 |
0.384599609 |
0.00005 |
2.493019545 |
2.122380845
|
10 |
0.384599609 |
0.000025 |
1.246509773 |
3.368890618
|
15 |
0.384599609 |
1.66667E-05 |
0.831006515 |
3.784393876
|
20 |
0.384599609 |
0.0000125 |
0.623254886 |
3.992145504
|
25 |
0.384599609 |
0.00001 |
0.498603909 |
4.116796482
|
30 |
0.384599609 |
8.33333E-06 |
0.415503258 |
4.199897133
|
35 |
0.384599609 |
7.14286E-06 |
0.356145649 |
4.259254741
|
40 |
0.384599609 |
0.00000625 |
0.311627443 |
4.303772947
|
45 |
0.384599609 |
5.55556E-06 |
0.277002172 |
4.338398219
|
50 |
0.384599609 |
0.000005 |
0.249301955 |
4.366098436
|
|