User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/11/13: Difference between revisions
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== | ==What was done== | ||
Today we analyzed our gold nanoparticles solutions using atomic absorption | |||
==Notes== | |||
Table 1. Standard Au Solution preparation from 1000μg/mL AA Au gold standard solution | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Concentration(μg/mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume of stock Au solution(mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume Deionized water added(mL)''' | |||
|- | |||
| 10||0.1||9.9 | |||
|- | |||
| 20||0.2||9.8 | |||
|- | |||
| 30||0.3||9.7 | |||
|- | |||
| 40||0.4||9.6 | |||
|- | |||
| 50||0.5||9.5 | |||
|} | |||
<br> | |||
Table 2. AA spectroscopy AuNP-protein solutions observations | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Au:Lysozyme Ratio''' | |||
| align="center" style="background:#f0f0f0;"|'''Appearance of solution''' | |||
| align="center" style="background:#f0f0f0;"|'''Au:Hemoglobin Ratio''' | |||
| align="center" style="background:#f0f0f0;"|'''Appearance of solution''' | |||
|- | |||
| 5||Colorless||30||Lightly colored | |||
|- | |||
| 10||Lightly colored||50||Lightly colored | |||
|- | |||
| 15||Colored darkly||70||Darkly colored | |||
|- | |||
| 20||Colored||90||Darkly colored | |||
|- | |||
| 25||Colored||110||Colorless | |||
|- | |||
| 30||Colored||130||Colorless | |||
|- | |||
| 35||Colored||150||Colorless | |||
|- | |||
| 40||Colorless||170||Colorless | |||
|- | |||
| 45||Colorless||190||Colorless | |||
|- | |||
| 50||Colorless||210||Colorless | |||
|} | |||
<br> | |||
*All of the solutions, if stated as colored, had a purplish color. | |||
<br> | |||
Figure 1. Absorbance spectra of AuNP Lysozyme solutions | |||
<br> | |||
[[Image:Atomic Absorbance Spectra of AuNP Lysozyme solutions zem .png]] | |||
<br> | |||
Figure 2. Absorbance Spectra of AuNP-Hemoglobin solutions | |||
<br> | |||
[[Image:Atomic Absorbance Spectra of AuNP-Hemoglobin solutions zem.png]] | |||
<Br> | |||
Figure 3. Calibration Curve for Gold Standards | |||
<br> | |||
[[Image:Calibration Curve for Gold Standards lysozymehemozem.png]] | |||
<br> | |||
Figure 4. Concentration vs. Absorbance Au-Hemoglobin solutions | |||
[[Image:Concentration vs. Absorbance AuNP-Hemoglobin solutions zem.png]] | |||
<br> | |||
Figure 5. Concentration vs. Absrobance AuNP-Lysozyme solutions | |||
<br> | |||
[[Image:Concentration vs. Absorbance of AuNP-Lysozyme solutions zem.png]] | |||
<br> | |||
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Revision as of 15:12, 1 December 2013
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What was doneToday we analyzed our gold nanoparticles solutions using atomic absorption NotesTable 1. Standard Au Solution preparation from 1000μg/mL AA Au gold standard solution
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