User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/11/13

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==Notes==
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Table 1. Standard Au Solution preparation from 1000μg/mL AA Au gold standard solution
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Concentration(μg/mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume of stock Au solution(mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Deionized water added(mL)'''
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|-
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| 10||0.1||9.9
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|-
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| 20||0.2||9.8
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|-
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| 30||0.3||9.7
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|-
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| 40||0.4||9.6
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|-
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| 50||0.5||9.5
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|}
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<br>
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Table 2. AA spectroscopy AuNP-protein solutions observations
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Au:Lysozyme Ratio'''
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| align="center" style="background:#f0f0f0;"|'''Appearance of solution'''
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| align="center" style="background:#f0f0f0;"|'''Au:Hemoglobin Ratio'''
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| align="center" style="background:#f0f0f0;"|'''Appearance of solution'''
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|-
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| 5||Colorless||30||Lightly colored
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|-
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| 10||Lightly colored||50||Lightly colored
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|-
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| 15||Colored darkly||70||Darkly colored
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|-
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| 20||Colored||90||Darkly colored
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|-
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| 25||Colored||110||Colorless
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|-
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| 30||Colored||130||Colorless
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|-
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| 35||Colored||150||Colorless
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|-
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| 40||Colorless||170||Colorless
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|-
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| 45||Colorless||190||Colorless
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|-
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| 50||Colorless||210||Colorless
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|}
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<br>
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*All of the solutions, if stated as colored, had a purplish color.
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<br>
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Figure 1. Absorbance spectra of AuNP Lysozyme solutions
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<br>
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[[Image:Atomic Absorbance Spectra of AuNP Lysozyme solutions zem .png]]
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<br>
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Figure 2. Absorbance Spectra of AuNP-Hemoglobin solutions
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<br>
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[[Image:Atomic Absorbance Spectra of AuNP-Hemoglobin solutions zem.png]]
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<Br>
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Figure 3. Calibration Curve for Gold Standards
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<br>
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[[Image:Calibration Curve for Gold Standards lysozymehemozem.png]]
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<br>
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Figure 4. Concentration vs. Absorbance Au-Hemoglobin solutions
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[[Image:Concentration vs. Absorbance AuNP-Hemoglobin solutions zem.png]]
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<br>
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Figure 5. Concentration vs. Absrobance AuNP-Lysozyme solutions
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<br>
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[[Image:Concentration vs. Absorbance of AuNP-Lysozyme solutions zem.png]]
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<br>
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Revision as of 18:11, 1 December 2013

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Notes

Table 1. Standard Au Solution preparation from 1000μg/mL AA Au gold standard solution

Concentration(μg/mL) Volume of stock Au solution(mL) Volume Deionized water added(mL)
100.19.9
200.29.8
300.39.7
400.49.6
500.59.5


Table 2. AA spectroscopy AuNP-protein solutions observations

Au:Lysozyme Ratio Appearance of solution Au:Hemoglobin Ratio Appearance of solution
5Colorless30Lightly colored
10Lightly colored50Lightly colored
15Colored darkly70Darkly colored
20Colored90Darkly colored
25Colored110Colorless
30Colored130Colorless
35Colored150Colorless
40Colorless170Colorless
45Colorless190Colorless
50Colorless210Colorless


  • All of the solutions, if stated as colored, had a purplish color.


Figure 1. Absorbance spectra of AuNP Lysozyme solutions
Image:Atomic Absorbance Spectra of AuNP Lysozyme solutions zem .png
Figure 2. Absorbance Spectra of AuNP-Hemoglobin solutions
Image:Atomic Absorbance Spectra of AuNP-Hemoglobin solutions zem.png
Figure 3. Calibration Curve for Gold Standards
Image:Calibration Curve for Gold Standards lysozymehemozem.png
Figure 4. Concentration vs. Absorbance Au-Hemoglobin solutions Image:Concentration vs. Absorbance AuNP-Hemoglobin solutions zem.png
Figure 5. Concentration vs. Absrobance AuNP-Lysozyme solutions
Image:Concentration vs. Absorbance of AuNP-Lysozyme solutions zem.png


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