User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/11/19

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(Notes)
(Notes)
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| 50||0.5||9.5
| 50||0.5||9.5
|}
|}
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Table 2. Preparation of BSA stock solution
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of BSA stock solution'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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|Molecular weight (g/mol)||66000
 +
|-
 +
| mass (g)||0.0432
 +
|-
 +
| volume stock (L)||0.03
 +
|-
 +
| Concentration (M)||2.18182E-05
 +
|}
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<br>
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Table 3. Preparation of Invertase Stock solution
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Preparation Invertase Stock'''
 +
| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Molecular weight (g/mol)||135000
 +
|-
 +
| mass invertase (g)||0.1015
 +
|-
 +
| volume stock (L)||0.025
 +
|-
 +
| concentration (M)||3.00741E-05
 +
|}
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<br>
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Table 4. Preparation of BSA-AuNP solutions
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<br>
 +
{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
 +
| align="center" style="background:#f0f0f0;"|'''Amount of gold added(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''concentration BSA (M)'''
 +
| align="center" style="background:#f0f0f0;"|'''volume BSA (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''volume Water added (mL)'''
 +
|-
 +
| 105||0.347660664||2.38095E-06||0.545634921||4.106704416
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|-
 +
| 110||0.347660664||2.27273E-06||0.520833333||4.131506003
 +
|-
 +
| 115||0.347660664||2.17391E-06||0.498188406||4.15415093
 +
|-
 +
| 120||0.347660664||2.08333E-06||0.477430556||4.174908781
 +
|-
 +
| 125||0.347660664||0.000002||0.458333333||4.194006003
 +
|-
 +
| 130||0.347660664||1.92308E-06||0.440705128||4.211634208
 +
|-
 +
| 135||0.347660664||1.85185E-06||0.424382716||4.22795662
 +
|-
 +
| 140||0.347660664||1.78571E-06||0.40922619||4.243113146
 +
|-
 +
| 145||0.347660664||1.72414E-06||0.395114943||4.257224394
 +
|-
 +
| 150||0.347660664||1.66667E-06||0.381944444||4.270394892
 +
|}
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<br>
 +
Table 5. Preparation of Invertase-AuNP solutions
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Ratio'''
 +
| align="center" style="background:#f0f0f0;"|'''Amount gold added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Concentration Invertase (M)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Invertase (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume water added (mL)'''
 +
|-
 +
| 30||0.347660664||8.33333E-06||1.38546798||3.266871356
 +
|-
 +
| 50||0.347660664||0.000005||0.831280788||3.821058548
 +
|-
 +
| 70||0.347660664||3.57143E-06||0.593771992||4.058567345
 +
|-
 +
| 90||0.347660664||2.77778E-06||0.46182266||4.190516676
 +
|-
 +
| 110||0.347660664||2.27273E-06||0.377854904||4.274484432
 +
|-
 +
| 130||0.347660664||1.92308E-06||0.31972338||4.332615956
 +
|-
 +
| 150||0.347660664||1.66667E-06||0.277093596||4.37524574
 +
|-
 +
| 170||0.347660664||1.47059E-06||0.244494349||4.407844987
 +
|-
 +
| 190||0.347660664||1.31579E-06||0.218758102||4.433581234
 +
|-
 +
| 210||0.347660664||1.19048E-06||0.197923997||4.454415339
 +
|}
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<br>
 +
__NOTOC__
__NOTOC__

Revision as of 17:19, 1 December 2013

Project name Main project page
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Entry title

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Procedure

New Nanoparticle Synthesis: Please choose two from the following sets!

  1. Bovine serum albumin
    1. Ratios
      1. 105
      2. 110
      3. 115
      4. 120
      5. 125
      6. 130
      7. 135
      8. 140
      9. 145
      10. 150
  2. Catalase
    1. Ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210
  3. Invertase
    1. Ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210
  4. Elastin
    1. Ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210

Notes

Table 1. Preparation of Gold Standards

Concentration(μg/mL) Volume of stock Au solution(mL) Volume Deionized water added(mL)
100.19.9
200.29.8
300.39.7
400.49.6
500.59.5


Table 2. Preparation of BSA stock solution

Preparation of BSA stock solution '
Molecular weight (g/mol)66000
mass (g)0.0432
volume stock (L)0.03
Concentration (M)2.18182E-05


Table 3. Preparation of Invertase Stock solution

Preparation Invertase Stock '
Molecular weight (g/mol)135000
mass invertase (g)0.1015
volume stock (L)0.025
concentration (M)3.00741E-05


Table 4. Preparation of BSA-AuNP solutions

Ratio Amount of gold added(mL) concentration BSA (M) volume BSA (mL) volume Water added (mL)
1050.3476606642.38095E-060.5456349214.106704416
1100.3476606642.27273E-060.5208333334.131506003
1150.3476606642.17391E-060.4981884064.15415093
1200.3476606642.08333E-060.4774305564.174908781
1250.3476606640.0000020.4583333334.194006003
1300.3476606641.92308E-060.4407051284.211634208
1350.3476606641.85185E-060.4243827164.22795662
1400.3476606641.78571E-060.409226194.243113146
1450.3476606641.72414E-060.3951149434.257224394
1500.3476606641.66667E-060.3819444444.270394892


Table 5. Preparation of Invertase-AuNP solutions

Ratio Amount gold added (mL) Concentration Invertase (M) Volume Invertase (mL) Volume water added (mL)
300.3476606648.33333E-061.385467983.266871356
500.3476606640.0000050.8312807883.821058548
700.3476606643.57143E-060.5937719924.058567345
900.3476606642.77778E-060.461822664.190516676
1100.3476606642.27273E-060.3778549044.274484432
1300.3476606641.92308E-060.319723384.332615956
1500.3476606641.66667E-060.2770935964.37524574
1700.3476606641.47059E-060.2444943494.407844987
1900.3476606641.31579E-060.2187581024.433581234
2100.3476606641.19048E-060.1979239974.454415339



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