User:Zachary I. Mendel/Notebook/Zacks Notebook/2014/02/05: Difference between revisions
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== | ==February 5th== | ||
==Procedure== | ==Procedure== | ||
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#Add a 0.5mL of the citrate sodium solution and reflux for 30 minutes | #Add a 0.5mL of the citrate sodium solution and reflux for 30 minutes | ||
##Note color change from dark blue to red | ##Note color change from dark blue to red | ||
*Protein Gold Nanoparticles | |||
**First I made the buffer solution. The pH was 5. I made it using 0.0003 moles of acetic acid 1M and 0.0006 moles of potassium acetate | |||
**Going to make 10^-6M HRP solution in the buffer\ | |||
***we then filled up 100ml volumetric flask | |||
**Take 1ml of the solution and add to 9ml of nanoparticles...take 100ul and add to 9.9mls of gold to make solutions | |||
<br> | |||
*one solution was 10^-7M protein in nanoparticles. The other was 10^-8 | |||
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Revision as of 11:33, 17 April 2014
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February 5thProcedure
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