User:Zachary I. Mendel/Notebook/Zacks Notebook/2014/02/18: Difference between revisions
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*one solution was 10^-7M protein in nanoparticles. The other was 10^-8 | *one solution was 10^-7M protein in nanoparticles. The other was 10^-8 | ||
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measure activity of HRP nanoparticles | |||
#HRP-AuNP complex solution from the alpha method and the polydopamine-coated AuNPs were placed in high velocity tubes. | |||
#The nano particle solutions were spun at 18,000rpm for 3 hours. | |||
#The supernatants were discarded and the pellets were resuspended in water. | |||
#The polydopamine-coated AuNPs were stored at 4 degrees Celsius | |||
#The resuspended HRP-AuNP complex solutions were analyzed for activity using UV-Vis | |||
##The nano particles were added to hydrogen peroxide and OPD (Ophenylenediamine) | |||
##Active HRP catalyzes the reaction of Ophenylenediamine with H2O2 to produce 2,3-Diaminophenazine | |||
[[Image:Screen Shot 2014-02-16 at 10.44.57 PM.png|400px]] | |||
###Stock solution of OPD with a concentration of 1mg/mL | |||
###Stock solution of H<sub>2</sub>O<sub>2</sub> 113uL | |||
==Notes== | ==Notes== |
Revision as of 11:32, 25 February 2014
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