April 8th
Goals
- spin down HRP nanoparticles and resuspend pellets
- these are the nanoparticles that have already been spun down once
- we will then run UV-Vis on the samples
- we will analyze the data and compare which nanoparticles have more activity
procedure
notes
- After we spun down our HRP nanoparticles for SPIN 2 we added some of the supernatant to OPD and H202
- The color change for this was almost instantaneous
- This was a little weird as the supernatants did not turn as fast the first time we way back when we tested HRP nanoparticles.
- Differences between this batch and the other batch
- More gold nanoparticles in this batch than previous
- This was after second spin
- this could mean there was more time for them to fall into solution
- The time difference alone between attachment could show that the particles are not staying attached.
- we are doing AA to see if there is gold in the supernatant
- We want to see if the gold nanoparticles made it into the supernatant somehow
- when we did AA before we saw that the gold was in pellet not the sup
- Molar Mass Hemoglobin is 17kDa...I need to add 0.0017g
- Molar mass myoglobin is 65kDa...I need to add 0.0065g
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