User talk:Anthony Salvagno: Difference between revisions
Andy Maloney (talk | contribs) (New page: ~~~~: So [http://jds.fass.org/cgi/reprint/71/1/29.pdf this] is the paper I found that talks about making E. coli produce cow utter puss. I can't understand any of it but if we do end up us...) |
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==Re: Dunn Lab logo== | |||
*[[User:Ramalldf|Ramalldf]] 20:03, 30 October 2009 (EDT):I made it a few months ago. Send it over, the reason that I used that font was cause I used the MASH font to make it look top secret for the private wiki logo so I just did the same but changed the colors. | |||
==Capping hairpin 2== | |||
[[User:Steven J. Koch|Steve Koch]] 12:38, 11 August 2009 (EDT): OK, here's a google doc with my notes, and hopefully the same sequence as in the note below: | |||
{{ShowGoogleWord|id=0ARLNnjMk2r_qZGdxamtoNnBfMTE0ZDlmeDhjZjM|width=500|height=300}} | |||
==Capping hairpin== | |||
Here is the hairpin I used in grad school: | |||
<pre> | |||
Unbiotinylated end-cap, HindIII | |||
5’-P-TCGAGGCGACCTAGCGTAAGCTAGGTCGCC | |||
Name = P16-Cap-HindIII(incorrect!!!!) | |||
L=30 | |||
Purification = HPLC | |||
Modifications = 5’ phosphate for ligation | |||
</pre> | |||
I think you should: | |||
* Check with m-fold that it's a hairpin | |||
* replace the 3 or 4 base sticky end with 3 or 4 based for SapI that you have | |||
==GRD $$== | |||
[[User:Andy Maloney|Andy Maloney]] 11:16, 7 May 2009 (EDT): So Larry thinks I'm nagging about the GRD monies. I know I am but, I just cannot stop thinking about what we should get. And, since we are getting just about everything for the Kiney experiments really quickly, I think we should act on this sooner rather than later. I know you said you wanted me and Larry to tell you what you should get so here is what we came up with. | |||
'''Water purifier''' | |||
* [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=80078-878&inE=1&highlight=80078-878&from_search=1 Cartridge Kit] | |||
* [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=26303-500&inE=1&highlight=26303-500&from_search=1 26303-500] Sanitation Kit. | |||
* [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=80078-876&inE=1&highlight=80078-876&from_search=1 Start Up Kit] | |||
* [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=80078-880&inE=1&highlight=80078-880&from_search=1 Booster Pump] | |||
* [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=80078-882&inE=1&highlight=80078-882&from_search=1 Prefilter Kit] | |||
'''pH Meter''' | |||
* [http://www.denverinstrumentusa.com/closeout/closeout2.php?catID=200 Model 250]. Get the complete kit. | |||
'''Update''' | |||
Actually, I found a more all purpose meter from VWR. | |||
* [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=11388-328&inE=1&highlight=11388-328 pH meter]. | |||
It has software that comes with it and it comes with 3 probes for pH, conductivity, and dissolved oxygen content. Evans has an osmometer but hey, this may work for us. I'll get a quote for the stuff. | |||
We will need to get pH standards for the meter and possibly conductivity standards but CRLS has those. I have a quote for the water filter stuff that is not listed on VWR's site so you are left with about $150 which should cover shipping charges for this stuff. | |||
Can you let me know when you will be in the lab next? I think we should have a Koch Lab meeting soon. | |||
==Recombinant Casein Paper== | |||
[[User:Andy Maloney|Andy Maloney]] 16:42, 24 April 2009 (EDT): So [http://jds.fass.org/cgi/reprint/71/1/29.pdf this] is the paper I found that talks about making E. coli produce cow utter puss. I can't understand any of it but if we do end up using <math>\kappa</math> casein a lot, we may want to make our own. If you are up for a challenge and read this, can you explain it to me? | [[User:Andy Maloney|Andy Maloney]] 16:42, 24 April 2009 (EDT): So [http://jds.fass.org/cgi/reprint/71/1/29.pdf this] is the paper I found that talks about making E. coli produce cow utter puss. I can't understand any of it but if we do end up using <math>\kappa</math> casein a lot, we may want to make our own. If you are up for a challenge and read this, can you explain it to me? |
Latest revision as of 21:13, 12 March 2010
Re: Dunn Lab logo
- Ramalldf 20:03, 30 October 2009 (EDT):I made it a few months ago. Send it over, the reason that I used that font was cause I used the MASH font to make it look top secret for the private wiki logo so I just did the same but changed the colors.
Capping hairpin 2
Steve Koch 12:38, 11 August 2009 (EDT): OK, here's a google doc with my notes, and hopefully the same sequence as in the note below: {{#widget:Google Documents |key=0ARLNnjMk2r_qZGdxamtoNnBfMTE0ZDlmeDhjZjM |width=500 |height=300 }}
Capping hairpin
Here is the hairpin I used in grad school:
Unbiotinylated end-cap, HindIII 5’-P-TCGAGGCGACCTAGCGTAAGCTAGGTCGCC Name = P16-Cap-HindIII(incorrect!!!!) L=30 Purification = HPLC Modifications = 5’ phosphate for ligation
I think you should:
- Check with m-fold that it's a hairpin
- replace the 3 or 4 base sticky end with 3 or 4 based for SapI that you have
GRD $$
Andy Maloney 11:16, 7 May 2009 (EDT): So Larry thinks I'm nagging about the GRD monies. I know I am but, I just cannot stop thinking about what we should get. And, since we are getting just about everything for the Kiney experiments really quickly, I think we should act on this sooner rather than later. I know you said you wanted me and Larry to tell you what you should get so here is what we came up with.
Water purifier
- Cartridge Kit
- 26303-500 Sanitation Kit.
- Start Up Kit
- Booster Pump
- Prefilter Kit
pH Meter
- Model 250. Get the complete kit.
Update
Actually, I found a more all purpose meter from VWR.
It has software that comes with it and it comes with 3 probes for pH, conductivity, and dissolved oxygen content. Evans has an osmometer but hey, this may work for us. I'll get a quote for the stuff.
We will need to get pH standards for the meter and possibly conductivity standards but CRLS has those. I have a quote for the water filter stuff that is not listed on VWR's site so you are left with about $150 which should cover shipping charges for this stuff.
Can you let me know when you will be in the lab next? I think we should have a Koch Lab meeting soon.
Recombinant Casein Paper
Andy Maloney 16:42, 24 April 2009 (EDT): So this is the paper I found that talks about making E. coli produce cow utter puss. I can't understand any of it but if we do end up using [math]\displaystyle{ \kappa }[/math] casein a lot, we may want to make our own. If you are up for a challenge and read this, can you explain it to me?