User talk:Brian P. Josey/Notebook/2009/08/28: Difference between revisions

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(New page: ~~~~: Thanks, Ant, for teaching Brian! Here's my take (you can develop your own technique in time): * For the same sized gel, I used 25 ml * 0.8% is probably better for the longer fragmen...)
 
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* 0.8% is probably better for the longer fragments that we're typically looking at
* 0.8% is probably better for the longer fragments that we're typically looking at
* I didn't add back missing water.  Not sure, though, if this is more of a problem in ABQ than Ithaca
* I didn't add back missing water.  Not sure, though, if this is more of a problem in ABQ than Ithaca
[[User:Brian P. Josey|Brian Josey]] 10:37, 28 August 2009 (EDT): Thanks for the advice. Anthony said that I do not have to replace the water that evaporates off, but I wrote down that step just in case it ever became a problem.

Latest revision as of 07:39, 31 August 2009

Steve Koch 23:37, 28 August 2009 (EDT): Thanks, Ant, for teaching Brian! Here's my take (you can develop your own technique in time):

  • For the same sized gel, I used 25 ml
  • 0.8% is probably better for the longer fragments that we're typically looking at
  • I didn't add back missing water. Not sure, though, if this is more of a problem in ABQ than Ithaca

Brian Josey 10:37, 28 August 2009 (EDT): Thanks for the advice. Anthony said that I do not have to replace the water that evaporates off, but I wrote down that step just in case it ever became a problem.