User talk:Emilio Palumbo/g2f rna

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(New page: ==RNAseq== link= ===Mapping=== Specific variables to consider when mapping RNAseq: * intron size * overhang (number of bases from each...)
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Revision as of 04:34, 15 November 2013

RNAseq

link=

Mapping

Specific variables to consider when mapping RNAseq:

  • intron size
  • overhang (number of bases from each side of the junction that should be covered by a certain read)
  • splice site consensus (canonical, extended, non-canonical)
  • donor/acceptor splice site consensus sequences
  • junction “filtering”:
    • chromosome/strand
    • block order
    • min/max distance
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