User talk:Emilio Palumbo/g2f rna: Difference between revisions
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(New page: ==RNAseq== link= ===Mapping=== Specific variables to consider when mapping RNAseq: * intron size * overhang (number of bases from each...) |
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Revision as of 01:34, 15 November 2013
RNAseq
File:Http://rnaseq.uoregon.edu/img/image1.png
Mapping
Specific variables to consider when mapping RNAseq:
- intron size
- overhang (number of bases from each side of the junction that should be covered by a certain read)
- splice site consensus (canonical, extended, non-canonical)
- donor/acceptor splice site consensus sequences
- junction “filtering”:
- chromosome/strand
- block order
- min/max distance