User talk:Federico Castro M: Difference between revisions
No edit summary |
No edit summary |
||
| (5 intermediate revisions by 2 users not shown) | |||
| Line 4: | Line 4: | ||
Feel free to post. | Feel free to post. | ||
[[Image:Scientificwriters.png]] | |||
---- | |||
'''[[User:Jason R. Kelly|Jason R. Kelly]] 02:07, 17 January 2008 (CST):''' | |||
Hi Frederico, | |||
I'm a grad student in drew endy's lab, was taking a look at your [[FedericoC Repressilator|repressilator project]]. You might consider using a different CFP then the one contained in <bbpart>E0422</bbpart>. Unfortunately, the ssrA tag on <bbpart>E0022</bbpart> is LVA and that's stronger than the tag used by Elowitz. It's so strong that you basically get no detectable signal since the FPs get degraded so quickly (even when behind a very strong promoter). I'd recommend going with the same tag as elowitz if you're going to give this a try, or at least a weaker tag than LVA. | |||
Thanks for posting the project up. Also, I saw you're keeping a lab notebook on OWW -- we're rolling out a new lab notebook tool that you might want to try out. you can sign up for the SC mailing list to keep tabs on it: | |||
http://mailman.mit.edu/mailman/listinfo/oww-sc | |||
We'll also post something on the SC blog when it comes out. | |||
http://blog.openwetware.org/sc/ | |||
good luck, | |||
jason | |||
Latest revision as of 21:41, 30 January 2008
Welcome visitor!
This is a place for you to comment, ask or discuss anything about my work.
Feel free to post.
Jason R. Kelly 02:07, 17 January 2008 (CST):
Hi Frederico,
I'm a grad student in drew endy's lab, was taking a look at your repressilator project. You might consider using a different CFP then the one contained in <bbpart>E0422</bbpart>. Unfortunately, the ssrA tag on <bbpart>E0022</bbpart> is LVA and that's stronger than the tag used by Elowitz. It's so strong that you basically get no detectable signal since the FPs get degraded so quickly (even when behind a very strong promoter). I'd recommend going with the same tag as elowitz if you're going to give this a try, or at least a weaker tag than LVA.
Thanks for posting the project up. Also, I saw you're keeping a lab notebook on OWW -- we're rolling out a new lab notebook tool that you might want to try out. you can sign up for the SC mailing list to keep tabs on it: http://mailman.mit.edu/mailman/listinfo/oww-sc
We'll also post something on the SC blog when it comes out. http://blog.openwetware.org/sc/
good luck, jason
