User talk:John Cumbers: Difference between revisions

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I noticed your comment about caps when dewikifying ISCB-SC. The default assumes only the first character is capitalized. But we can easily accomodate any capitalization or even completely different names as aliases as written on [[OpenWetWare:Dewikify]]. I'd recommend using whatever capitalization makes most sense for you on the wiki, and we can handle the dewikifying separately. --[[User:Austin|Austin]] 18:15, 15 January 2006 (EST)
'''[[User:Lucks|Lucks]] 17:02, 25 July 2006 (EDT)''': How about we skype sometime on Friday?


Hey John, Not sure if you noticed but I merged your [[Tips and templates]] page with the previously existing [[OpenWetWare:Tips]] page and made Tips and templates a redirect. I figured this would keep everything in one spot making stuff easier to find. Nice template BTW!  --[[User:Rshetty|RS]]
'''[[User:Lucks|Lucks]] 13:37, 25 July 2006 (EDT)'''': John, Let's find a way to chat more about how OWW or something like OWW can change the current publishing model.
 
== DNA extraction ==
 
Hi John. The ratios are definitely crap, but even so, I have found this protocol very robust for generating PCR templates. In addition to regular PCR, I have used the DNA in quantitative PCR in a dilution series to calibrate different sets of primers and the standard curve was as tight as a dilution of cDNA. I have not used the DNA for anything else. Cheers, Michael
 
== the torch is passed ;) ==
[[User:John Cumbers/Torch]]

Latest revision as of 21:34, 18 March 2008

Lucks 17:02, 25 July 2006 (EDT): How about we skype sometime on Friday?

Lucks 13:37, 25 July 2006 (EDT)': John, Let's find a way to chat more about how OWW or something like OWW can change the current publishing model.

DNA extraction

Hi John. The ratios are definitely crap, but even so, I have found this protocol very robust for generating PCR templates. In addition to regular PCR, I have used the DNA in quantitative PCR in a dilution series to calibrate different sets of primers and the standard curve was as tight as a dilution of cDNA. I have not used the DNA for anything else. Cheers, Michael

the torch is passed ;)

User:John Cumbers/Torch

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