User talk:John Cumbers: Difference between revisions

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Hi John, I added <html> tags to your video and it seems to work now on Firefox.  However, I am definitely not in the way.
'''[[User:Lucks|Lucks]] 17:02, 25 July 2006 (EDT)''': How about we skype sometime on Friday?


John, you should thank Barry for that. He beat me to it. In any case, I'm not sure we want to leave the html tag turned on as it's a supposed security risk allowing arbitrary HTML. I'm looking at alternatives so I wouldn't use it too much yet. And yes, you get an email when anyone leaves a message here automatically. --[[User:Austin|Austin]] 20:12, 1 March 2006 (EST)
'''[[User:Lucks|Lucks]] 13:37, 25 July 2006 (EDT)'''': John, Let's find a way to chat more about how OWW or something like OWW can change the current publishing model.


I don't know what's popping up for you. I don't get anything popping up. Anyway, you'd have to ask Barry what he meant with "definitely not in the way." --[[User:Austin|Austin]] 20:25, 1 March 2006 (EST)
== DNA extraction ==


==Highlights==
Hi John. The ratios are definitely crap, but even so, I have found this protocol very robust for generating PCR templates. In addition to regular PCR, I have used the DNA in quantitative PCR in a dilution series to calibrate different sets of primers and the standard curve was as tight as a dilution of cDNA. I have not used the DNA for anything else. Cheers, Michael
*'''[[User:Jennyn|Jennyn]] 18:59, 19 March 2006 (EST)''': Awesome work on the highlights! I like the idea that we use the lecturers picture rather than an icon. You rock! [[Image:Smiley.gif]] Incase you run into a time that you need a "lecture" icon, here's [http://openwetware.org/images/c/c6/Lecture.png one].


*'''[[User:Jennyn|Jennyn]] 23:30, 19 March 2006 (EST)''': Oh, incase you didn't get the message I left you on one of the discussions a while ago, whenever you change the highlights (every month or two weeks or however you plan to do this), please change ALL FOUR of the icons. We've added the Getting Started and Recent Changes icons to the navigation bar and Meta section. Woot!
== the torch is passed ;) ==
 
[[User:John Cumbers/Torch]]
== BioSysBio abstracts ==
 
'''[[User:Skosuri|Sri Kosuri]] 17:16, 28 March 2006 (EST)''':Just highlight your discussion on the community portal wherever you think it is appropriate.

Latest revision as of 21:34, 18 March 2008

Lucks 17:02, 25 July 2006 (EDT): How about we skype sometime on Friday?

Lucks 13:37, 25 July 2006 (EDT)': John, Let's find a way to chat more about how OWW or something like OWW can change the current publishing model.

DNA extraction

Hi John. The ratios are definitely crap, but even so, I have found this protocol very robust for generating PCR templates. In addition to regular PCR, I have used the DNA in quantitative PCR in a dilution series to calibrate different sets of primers and the standard curve was as tight as a dilution of cDNA. I have not used the DNA for anything else. Cheers, Michael

the torch is passed ;)

User:John Cumbers/Torch

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