User talk:John Cumbers: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
m (User talk:Johncumbers moved to User talk:John Cumbers: Automatically moved page while renaming the user "Johncumbers" to "John Cumbers") |
Mpovelones (talk | contribs) (DNA extraction) |
||
| Line 2: | Line 2: | ||
'''[[User:Lucks|Lucks]] 13:37, 25 July 2006 (EDT)'''': John, Let's find a way to chat more about how OWW or something like OWW can change the current publishing model. | '''[[User:Lucks|Lucks]] 13:37, 25 July 2006 (EDT)'''': John, Let's find a way to chat more about how OWW or something like OWW can change the current publishing model. | ||
== DNA extraction == | |||
Hi John. The ratios are definitely crap, but even so, I have found this protocol very robust for generating PCR templates. In addition to regular PCR, I have used the DNA in quantitative PCR in a dilution series to calibrate different sets of primers and the standard curve was as tight as a dilution of cDNA. I have not used the DNA for anything else. Cheers, Michael | |||
Revision as of 10:15, 24 January 2008
Lucks 17:02, 25 July 2006 (EDT): How about we skype sometime on Friday?
Lucks 13:37, 25 July 2006 (EDT)': John, Let's find a way to chat more about how OWW or something like OWW can change the current publishing model.
DNA extraction
Hi John. The ratios are definitely crap, but even so, I have found this protocol very robust for generating PCR templates. In addition to regular PCR, I have used the DNA in quantitative PCR in a dilution series to calibrate different sets of primers and the standard curve was as tight as a dilution of cDNA. I have not used the DNA for anything else. Cheers, Michael
