User talk:John Cumbers: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Mpovelones (talk | contribs) (DNA extraction) |
No edit summary |
||
| Line 6: | Line 6: | ||
Hi John. The ratios are definitely crap, but even so, I have found this protocol very robust for generating PCR templates. In addition to regular PCR, I have used the DNA in quantitative PCR in a dilution series to calibrate different sets of primers and the standard curve was as tight as a dilution of cDNA. I have not used the DNA for anything else. Cheers, Michael | Hi John. The ratios are definitely crap, but even so, I have found this protocol very robust for generating PCR templates. In addition to regular PCR, I have used the DNA in quantitative PCR in a dilution series to calibrate different sets of primers and the standard curve was as tight as a dilution of cDNA. I have not used the DNA for anything else. Cheers, Michael | ||
== the torch is passed ;) == | |||
[[User:John Cumbers/Torch]] | |||
Latest revision as of 21:34, 18 March 2008
Lucks 17:02, 25 July 2006 (EDT): How about we skype sometime on Friday?
Lucks 13:37, 25 July 2006 (EDT)': John, Let's find a way to chat more about how OWW or something like OWW can change the current publishing model.
DNA extraction
Hi John. The ratios are definitely crap, but even so, I have found this protocol very robust for generating PCR templates. In addition to regular PCR, I have used the DNA in quantitative PCR in a dilution series to calibrate different sets of primers and the standard curve was as tight as a dilution of cDNA. I have not used the DNA for anything else. Cheers, Michael
