User talk:John Cumbers

Lucks 17:02, 25 July 2006 (EDT): How about we skype sometime on Friday?

Lucks 13:37, 25 July 2006 (EDT)': John, Let's find a way to chat more about how OWW or something like OWW can change the current publishing model.

DNA extraction

Hi John. The ratios are definitely crap, but even so, I have found this protocol very robust for generating PCR templates. In addition to regular PCR, I have used the DNA in quantitative PCR in a dilution series to calibrate different sets of primers and the standard curve was as tight as a dilution of cDNA. I have not used the DNA for anything else. Cheers, Michael