User talk:Nkuldell/SAGA swap: Difference between revisions
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[[Image:Macintosh HD-Users-nkuldell-Desktop-SGF73alignment1.jpg|thumb|400 px|right]] | [[Image:Macintosh HD-Users-nkuldell-Desktop-SGF73alignment1.jpg|thumb|400 px|right]] | ||
[[Image:Macintosh HD-Users-nkuldell-Desktop-SGF73align2.jpg|thumb|400 px|right]] <br> | [[Image:Macintosh HD-Users-nkuldell-Desktop-SGF73align2.jpg|thumb|400 px|right]] <br> | ||
Notes from discussion with Dom: | |||
**middle block of alignment has non-canonical Zn finger that is conserved with human SGF73 and that he showed by mass spec can bind Zn. This block is exquisitely conserved across species. | |||
**first block of alignment has homology to SGF11 as well, including a more traditional Zn finger. | |||
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Revision as of 13:28, 31 July 2006
Notes on SGF73 swap
H.s. to S.c. swap of SGF73
at [[1]] fill in paper details here...
alignment SGF73 S. pombe vs S. cerevisiae
protein alignment using blastp at [2]
SGF73 protein in S. cerevisiae is 657 aa
SPCC126.04c protein in S. pombe is 344 aa
Notes from discussion with Dom:
- middle block of alignment has non-canonical Zn finger that is conserved with human SGF73 and that he showed by mass spec can bind Zn. This block is exquisitely conserved across species.
- first block of alignment has homology to SGF11 as well, including a more traditional Zn finger.
codon usage SGF73 S. pombe vs S. cerevisiae
Codon Usage Comparison using GeneArt tool called Graphical Codon Usage Analyzer [3]
alignment SGF29 S. pombe vs S. cerevisiae
protein alignment using blastp at [4]
SGF29 protein in S. cerevisiae is 259aa
SPBC1921.07c protein in S. pombe is 244 aa
