User talk:Nkuldell/SAGA swap: Difference between revisions

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===H.s. to S.c. swap of SGF73===  
===H.s. to S.c. swap of SGF73===  
at [[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed]]
at [[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed]]
fill in paper details here...
fill in paper details here...<br>
and for Dom's paper [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=15115762&query_hl=5&itool=pubmed_docsum] <br>[[Image:Macintosh HD-Users-nkuldell-Desktop-HelmlingerHMG04 1.pdf]]




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===codon usage SGF73 S. pombe vs S. cerevisiae===
===codon usage SGF73 S. pombe vs S. cerevisiae===
Codon Usage Comparison using GeneArt tool called Graphical Codon Usage Analyzer [http://gcua.schoedl.de/sequential.php] <br>
Codon Usage Comparison using GeneArt tool called Graphical Codon Usage Analyzer [http://gcua.schoedl.de/sequential.php] <br>
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SPBC1921.07c protein in S. pombe is 244 aa <br>
SPBC1921.07c protein in S. pombe is 244 aa <br>
[[Image:Macintosh HD-Users-nkuldell-Desktop-BlastP SGF29 SpvSc.jpg|thumb|400 px|left]]
[[Image:Macintosh HD-Users-nkuldell-Desktop-BlastP SGF29 SpvSc.jpg|thumb|400 px|left]]
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===codon usage SGF29 S. pombe vs S. cerevisiae===
===codon usage SGF29 S. pombe vs S. cerevisiae===
Codon Usage Comparison using GeneArt tool called Graphical Codon Usage Analyzer [http://gcua.schoedl.de/sequential.php] <br>
Codon Usage Comparison using GeneArt tool [http://gcua.schoedl.de/sequential.php] <br>
[[Image:Macintosh HD-Users-nkuldell-Desktop-SGF29codonuse1.jpg|thumb|500 px|right]]<br>
[[Image:Macintosh HD-Users-nkuldell-Desktop-SGF29codonuse2.jpg|thumb|500 px|right]]<br>

Latest revision as of 18:53, 31 July 2006

Notes on SGF73 swap

H.s. to S.c. swap of SGF73

at [[1]] fill in paper details here...
and for Dom's paper [2]
File:Macintosh HD-Users-nkuldell-Desktop-HelmlingerHMG04 1.pdf


alignment SGF73 S. pombe vs S. cerevisiae

protein alignment using blastp at [3]
SGF73 protein in S. cerevisiae is 657 aa
SPCC126.04c protein in S. pombe is 344 aa


Notes from discussion with Dom:

    • middle block of alignment has non-canonical Zn finger that is conserved with human SGF73 and that he showed by mass spec can bind Zn. This block is exquisitely conserved across species.
    • first block of alignment has homology to SGF11 as well, including a more traditional Zn finger.




























codon usage SGF73 S. pombe vs S. cerevisiae

Codon Usage Comparison using GeneArt tool called Graphical Codon Usage Analyzer [4]























































alignment SGF29 S. pombe vs S. cerevisiae

protein alignment using blastp at [5]
SGF29 protein in S. cerevisiae is 259aa
SPBC1921.07c protein in S. pombe is 244 aa



















codon usage SGF29 S. pombe vs S. cerevisiae

Codon Usage Comparison using GeneArt tool [6]