User talk:Nkuldell/SAGA swap: Difference between revisions
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===H.s. to S.c. swap of SGF73=== | ===H.s. to S.c. swap of SGF73=== | ||
at [[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed]] | at [[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed]] | ||
fill in paper details here... | fill in paper details here...<br> | ||
and for Dom's paper [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=15115762&query_hl=5&itool=pubmed_docsum] <br>[[Image:Macintosh HD-Users-nkuldell-Desktop-HelmlingerHMG04 1.pdf]] | |||
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===codon usage SGF73 S. pombe vs S. cerevisiae=== | ===codon usage SGF73 S. pombe vs S. cerevisiae=== | ||
Codon Usage Comparison using GeneArt tool called Graphical Codon Usage Analyzer [http://gcua.schoedl.de/sequential.php] <br> | Codon Usage Comparison using GeneArt tool called Graphical Codon Usage Analyzer [http://gcua.schoedl.de/sequential.php] <br> | ||
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SPBC1921.07c protein in S. pombe is 244 aa <br> | SPBC1921.07c protein in S. pombe is 244 aa <br> | ||
[[Image:Macintosh HD-Users-nkuldell-Desktop-BlastP SGF29 SpvSc.jpg|thumb|400 px|left]] | [[Image:Macintosh HD-Users-nkuldell-Desktop-BlastP SGF29 SpvSc.jpg|thumb|400 px|left]] | ||
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===codon usage SGF29 S. pombe vs S. cerevisiae=== | ===codon usage SGF29 S. pombe vs S. cerevisiae=== | ||
Codon Usage Comparison using GeneArt tool | Codon Usage Comparison using GeneArt tool [http://gcua.schoedl.de/sequential.php] <br> | ||
[[Image:Macintosh HD-Users-nkuldell-Desktop-SGF29codonuse1.jpg|thumb|500 px|right]]<br> | |||
[[Image:Macintosh HD-Users-nkuldell-Desktop-SGF29codonuse2.jpg|thumb|500 px|right]]<br> |
Latest revision as of 18:53, 31 July 2006
Notes on SGF73 swap
H.s. to S.c. swap of SGF73
at [[1]]
fill in paper details here...
and for Dom's paper [2]
File:Macintosh HD-Users-nkuldell-Desktop-HelmlingerHMG04 1.pdf
alignment SGF73 S. pombe vs S. cerevisiae
protein alignment using blastp at [3]
SGF73 protein in S. cerevisiae is 657 aa
SPCC126.04c protein in S. pombe is 344 aa
Notes from discussion with Dom:
- middle block of alignment has non-canonical Zn finger that is conserved with human SGF73 and that he showed by mass spec can bind Zn. This block is exquisitely conserved across species.
- first block of alignment has homology to SGF11 as well, including a more traditional Zn finger.
codon usage SGF73 S. pombe vs S. cerevisiae
Codon Usage Comparison using GeneArt tool called Graphical Codon Usage Analyzer [4]
alignment SGF29 S. pombe vs S. cerevisiae
protein alignment using blastp at [5]
SGF29 protein in S. cerevisiae is 259aa
SPBC1921.07c protein in S. pombe is 244 aa
codon usage SGF29 S. pombe vs S. cerevisiae
Codon Usage Comparison using GeneArt tool [6]