User talk:Nkuldell/SAGA swap

Notes on SGF73 swap

H.s. to S.c. swap of SGF73

at [[1]] fill in paper details here...

alignment SGF73 S. pombe vs S. cerevisiae

protein alignment using blastp at [2]
SGF73 protein in S. cerevisiae is 657 aa
SPCC126.04c protein in S. pombe is 344 aa

Notes from discussion with Dom:

• • middle block of alignment has non-canonical Zn finger that is conserved with human SGF73 and that he showed by mass spec can bind Zn. This block is exquisitely conserved across species.
  • first block of alignment has homology to SGF11 as well, including a more traditional Zn finger.

codon usage SGF73 S. pombe vs S. cerevisiae

Codon Usage Comparison using GeneArt tool called Graphical Codon Usage Analyzer [3]

alignment SGF29 S. pombe vs S. cerevisiae

protein alignment using blastp at [4]
SGF29 protein in S. cerevisiae is 259aa
SPBC1921.07c protein in S. pombe is 244 aa

codon usage SGF29 S. pombe vs S. cerevisiae

Codon Usage Comparison using GeneArt tool called Graphical Codon Usage Analyzer [5]