User talk:Nkuldell/mtDNA pt3: Difference between revisions
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(New page: ==Experimental Details== ==mtRnt1== # regulation of expression from deoxycline-induced promoter, described in [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToS...) |
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==Experimental Details== | ==Experimental Details== | ||
==deoxycycline-regulation of RNase expression== | |||
[[Image:Macintosh HD-Users-nkuldell-Desktop-pCM224.PNG.PNG|thumb|plasmid map for pCM224]] | |||
# regulation of expression from deoxycline-induced promoter, described in [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=9778798&ordinalpos=4&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Herrero Yeast 1998 14(12):1127-38] | |||
#[[Media:Macintosh HD-Users-nkuldell-Desktop-mtRNA silencing-pCM224seq.rtf| sequence file]] for pCM224 which encodes tTA and CYC1-repressible promoter due to 2 copies of tetO | |||
# can excise relevant regulatory module (tTA driven by ADH1 and CYC1 with 2XtetO) on RI, BamHI fragment | |||
==mtRnt1== | ==mtRnt1== | ||
Revision as of 11:35, 26 June 2007
Experimental Details
deoxycycline-regulation of RNase expression
- regulation of expression from deoxycline-induced promoter, described in Herrero Yeast 1998 14(12):1127-38
- sequence file for pCM224 which encodes tTA and CYC1-repressible promoter due to 2 copies of tetO
- can excise relevant regulatory module (tTA driven by ADH1 and CYC1 with 2XtetO) on RI, BamHI fragment
