User talk:Nkuldell/mtDNA pt3

From OpenWetWare
Revision as of 11:35, 26 June 2007 by Nkuldell (talk | contribs)
Jump to navigationJump to search

Experimental Details

deoxycycline-regulation of RNase expression

plasmid map for pCM224
  1. regulation of expression from deoxycline-induced promoter, described in Herrero Yeast 1998 14(12):1127-38
  2. sequence file for pCM224 which encodes tTA and CYC1-repressible promoter due to 2 copies of tetO
  3. can excise relevant regulatory module (tTA driven by ADH1 and CYC1 with 2XtetO) on RI, BamHI fragment

mtRnt1