Van Oudenaarden Lab:C07H6.7

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(New page: Progress: Probes designed: (10-09-2009 - Ni Ji) Probes coupled: Oct 2009 Probes purified: Oct 2009 Probes tested: Oct 2009 Dyes available: Cy5 >K02B12.1 (lin-39 spliced coding region...)
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Dyes available: Cy5
Dyes available: Cy5
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 +
----
>K02B12.1 (lin-39 spliced coding region)
>K02B12.1 (lin-39 spliced coding region)
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TAAACCAATG ACACCTCCGA TGATGCCATT TGGTGCAAAT CTACCATTCG GTCCATTCCG GTTCCCACTT TTCAATCAAT  
TAAACCAATG ACACCTCCGA TGATGCCATT TGGTGCAAAT CTACCATTCG GTCCATTCCG GTTCCCACTT TTCAATCAAT  
TCTAG
TCTAG
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 +
----
Name of probe set: lin-39
Name of probe set: lin-39
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ggtgatgttgatgtggtcat, Probe_1, 1, 1, 45
ggtgatgttgatgtggtcat, Probe_1, 1, 1, 45
 +
tctcggtgcatctgtggatg, Probe_2, 2, 23, 55
tctcggtgcatctgtggatg, Probe_2, 2, 23, 55
 +
agcttgattcaggagctgta, Probe_3, 3, 45, 45
agcttgattcaggagctgta, Probe_3, 3, 45, 45
 +
gaagatgaggatgaagacga, Probe_4, 4, 67, 45
gaagatgaggatgaagacga, Probe_4, 4, 67, 45
 +
agaagatgtggatgatgatg, Probe_5, 5, 89, 40
agaagatgtggatgatgatg, Probe_5, 5, 89, 40
 +
atggaattccagatgcaccc, Probe_6, 6, 111, 50
atggaattccagatgcaccc, Probe_6, 6, 111, 50
 +
gtactcgataattcagaaga, Probe_7, 7, 133, 35
gtactcgataattcagaaga, Probe_7, 7, 133, 35
 +
gctgtcattggatcatatcc, Probe_8, 8, 157, 45
gctgtcattggatcatatcc, Probe_8, 8, 157, 45
 +
aatgagcagaaagtgcagca, Probe_9, 9, 180, 45
aatgagcagaaagtgcagca, Probe_9, 9, 180, 45
 +
gtcggatcataataacttcc, Probe_10, 10, 202, 40
gtcggatcataataacttcc, Probe_10, 10, 202, 40
 +
tgaagcaatttgagaagaac, Probe_11, 11, 224, 35
tgaagcaatttgagaagaac, Probe_11, 11, 224, 35
 +
gtccttgacttgaggcaaaa, Probe_12, 12, 246, 45
gtccttgacttgaggcaaaa, Probe_12, 12, 246, 45
 +
attggatattgaggacctcc, Probe_13, 13, 268, 45
attggatattgaggacctcc, Probe_13, 13, 268, 45
 +
gcatagtgactgatctccga, Probe_14, 14, 290, 50
gcatagtgactgatctccga, Probe_14, 14, 290, 50
 +
gtacttgttactgatggatt, Probe_15, 15, 313, 35
gtacttgttactgatggatt, Probe_15, 15, 313, 35
 +
caggtgcttccagtcgtgat, Probe_16, 16, 335, 55
caggtgcttccagtcgtgat, Probe_16, 16, 335, 55
 +
tatcatcatcatcgtctcct, Probe_17, 17, 357, 40
tatcatcatcatcgtctcct, Probe_17, 17, 357, 40
 +
atgcctttcttgtcatcatc, Probe_18, 18, 379, 40
atgcctttcttgtcatcatc, Probe_18, 18, 379, 40
 +
catatcatcgtcatcaccac, Probe_19, 19, 401, 45
catatcatcgtcatcaccac, Probe_19, 19, 401, 45
 +
ctgcaccgcctgaattctta, Probe_20, 20, 423, 50
ctgcaccgcctgaattctta, Probe_20, 20, 423, 50
 +
acacgtgtcatccatggata, Probe_21, 21, 445, 45
acacgtgtcatccatggata, Probe_21, 21, 445, 45
 +
tgaacctcctgtagttgaat, Probe_22, 22, 467, 40
tgaacctcctgtagttgaat, Probe_22, 22, 467, 40
 +
ttcgttgtcgcttctcgccg, Probe_23, 23, 489, 60
ttcgttgtcgcttctcgccg, Probe_23, 23, 489, 60
 +
acttgattccttgtgtatgc, Probe_24, 24, 511, 40
acttgattccttgtgtatgc, Probe_24, 24, 511, 40
 +
gaaattccttttccagctct, Probe_25, 25, 534, 40
gaaattccttttccagctct, Probe_25, 25, 534, 40
 +
ctcgtcagatatttgtgtgt, Probe_26, 26, 556, 40
ctcgtcagatatttgtgtgt, Probe_26, 26, 556, 40
 +
agctacttcaattctacgct, Probe_27, 27, 578, 40
agctacttcaattctacgct, Probe_27, 27, 578, 40
 +
tttcggtaagcatcaatgaa, Probe_28, 28, 600, 35
tttcggtaagcatcaatgaa, Probe_28, 28, 600, 35
 +
tgaaaccaaattttgacttg, Probe_29, 29, 622, 30
tgaaaccaaattttgacttg, Probe_29, 29, 622, 30
 +
tttgtgcttcattcgtcgat, Probe_30, 30, 644, 40
tttgtgcttcattcgtcgat, Probe_30, 30, 644, 40
 +
ggtgtcattggtttatcctt, Probe_31, 31, 673, 40
ggtgtcattggtttatcctt, Probe_31, 31, 673, 40
 +
tgcaccaaatggcatcatcg, Probe_32, 32, 695, 50
tgcaccaaatggcatcatcg, Probe_32, 32, 695, 50
 +
ggaatggaccgaatggtaga, Probe_33, 33, 717, 50
ggaatggaccgaatggtaga, Probe_33, 33, 717, 50
 +
gaattgattgaaaagtggga, Probe_34, 34, 740, 35
gaattgattgaaaagtggga, Probe_34, 34, 740, 35

Revision as of 18:18, 1 March 2010

Progress:

Probes designed: (10-09-2009 - Ni Ji)

Probes coupled: Oct 2009

Probes purified: Oct 2009

Probes tested: Oct 2009

Dyes available: Cy5


>K02B12.1 (lin-39 spliced coding region)

ATGACCA CATCAACATC ACCGTCATCC ACAGATGCAC CGAGAGCTAC AGCTCCTGAA TCAAGCTCTT CGTCTTCATC CTCATCTTCT TCATCATCAT CCACATCTTC TGTGGGTGCA TCTGGAATTC CATCATCTTC TGAATTATCG AGTACAATTG GATATGATCC AATGACAGCG TCTGCTGCAC TTTCTGCTCA TTTTGGAAGT TATTATGATC CGACTAGTTC TTCTCAAATT GCTTCATATT TTGCCTCAAG TCAAGGACTG GGAGGTCCTC AATATCCAAT ACTCGGAGAT CAGTCACTAT GCTATAATCC ATCAGTAACA AGTACCCATC ACGACTGGAA GCACCTGGAA GGAGACGATG ATGATGATAA GGATGATGAC AAGAAAGGCA TCAGTGGTGA TGACGATGAT ATGGATAAGA ATTCAGGCGG TGCAGTGTAT CCATGGATGA CACGTGTTCA TTCAACTACA GGAGGTTCAC GCGGCGAGAA GCGACAACGA ACAGCATACA CAAGGAATCA AGTATTAGAG CTGGAAAAGG AATTTCATAC ACACAAATAT CTGACGAGGA AGCGTAGAAT TGAAGTAGCT CATTCATTGA TGCTTACCGA AAGACAAGTC AAAATTTGGT TTCAAAATCG ACGAATGAAG CACAAAAAAG AAAATAAGGA TAAACCAATG ACACCTCCGA TGATGCCATT TGGTGCAAAT CTACCATTCG GTCCATTCCG GTTCCCACTT TTCAATCAAT TCTAG


Name of probe set: lin-39

Probes designed on 09 Oct 09

27 probes designed for target of length 762

Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC

ggtgatgttgatgtggtcat, Probe_1, 1, 1, 45

tctcggtgcatctgtggatg, Probe_2, 2, 23, 55

agcttgattcaggagctgta, Probe_3, 3, 45, 45

gaagatgaggatgaagacga, Probe_4, 4, 67, 45

agaagatgtggatgatgatg, Probe_5, 5, 89, 40

atggaattccagatgcaccc, Probe_6, 6, 111, 50

gtactcgataattcagaaga, Probe_7, 7, 133, 35

gctgtcattggatcatatcc, Probe_8, 8, 157, 45

aatgagcagaaagtgcagca, Probe_9, 9, 180, 45

gtcggatcataataacttcc, Probe_10, 10, 202, 40

tgaagcaatttgagaagaac, Probe_11, 11, 224, 35

gtccttgacttgaggcaaaa, Probe_12, 12, 246, 45

attggatattgaggacctcc, Probe_13, 13, 268, 45

gcatagtgactgatctccga, Probe_14, 14, 290, 50

gtacttgttactgatggatt, Probe_15, 15, 313, 35

caggtgcttccagtcgtgat, Probe_16, 16, 335, 55

tatcatcatcatcgtctcct, Probe_17, 17, 357, 40

atgcctttcttgtcatcatc, Probe_18, 18, 379, 40

catatcatcgtcatcaccac, Probe_19, 19, 401, 45

ctgcaccgcctgaattctta, Probe_20, 20, 423, 50

acacgtgtcatccatggata, Probe_21, 21, 445, 45

tgaacctcctgtagttgaat, Probe_22, 22, 467, 40

ttcgttgtcgcttctcgccg, Probe_23, 23, 489, 60

acttgattccttgtgtatgc, Probe_24, 24, 511, 40

gaaattccttttccagctct, Probe_25, 25, 534, 40

ctcgtcagatatttgtgtgt, Probe_26, 26, 556, 40

agctacttcaattctacgct, Probe_27, 27, 578, 40

tttcggtaagcatcaatgaa, Probe_28, 28, 600, 35

tgaaaccaaattttgacttg, Probe_29, 29, 622, 30

tttgtgcttcattcgtcgat, Probe_30, 30, 644, 40

ggtgtcattggtttatcctt, Probe_31, 31, 673, 40

tgcaccaaatggcatcatcg, Probe_32, 32, 695, 50

ggaatggaccgaatggtaga, Probe_33, 33, 717, 50

gaattgattgaaaagtggga, Probe_34, 34, 740, 35

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