Van Oudenaarden Lab:F22A3.5

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(New page: '''Progress:''' Probes designed: (03-01-2010 - Alexander van Oudenaarden) Probes coupled: Probes purified: Probes tested: Dyes available: ----)
Current revision (16:52, 1 March 2010) (view source)
 
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>F22A3.5 (ceh-60 spliced coding region)
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agacacatcacagctctgtgttgttgcgttcacaaccgagtgcatcacaccgatctttgagag
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atggataatcttattaagcagctccaaatgagcgtctcaagtgaaaacgtcgacaggaattttattgaaaaatgcatcga
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aatgaatccctgtcgccggatgcttcacgaagtgctacttgaaagaaaagacattttacgctctcggatgaacttggttg
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cgttgtatgatacagatgaacaatcctccgaaattgaggaaattctcgctcaattcgaaaatcctacatttccgttggaa
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gaggtcaacactgagaaagacgaggaatggcaacccttagaaaggaaatatttggacggaattaaccgtataaaagaaga
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cactatcatgaaacaaattcaactggacaaggatatggaaaaagccctgactaatagcgaagaagtactcagaaatcatc
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gcgattttagaccgatcgacgaaaaggatttctctaacattcggcaaagcatttccaaaaggttcgagcattcgaaaaat
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aacattcgcggggaggcagcaactaaaattctagttctccggagggagatcgagcagcagggacgcaagcgccggaactt
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tgacaaaaacacgacggacattttgcaaaactggttccacgatcatcgtcaaaatccttacccgtccgaccaggaaaagg
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cggaattagcaaaacagtgtaatatcaaaatttcacaggttaacaattggtttggaaatcaacgaattcggacaaaacaa
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caagctttgcgtatgcaagaagatgaacgtgaaagagctgcatcaatggctaacgaagcacaagcaattcagaattcttt
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gaacagttctacggttgcatcttcgtcgaatatgagcacatcggggctcattaacattccgctggttaacccaacaatgc
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aatcaatggtaattcctgcagtgcaaccaggcctacttggcaatccaaacggtcagttataa
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----
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Name of probe set: ceh-60
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Probes designed on Mon, 01 Mar 10 13:51:37 -0700
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45 probes designed for target of length 1005 (target too short for 48 probes)
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Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC
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cacagagctgtgatgtgtct, ceh-60_1, 1, 1, 50
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cactcggttgtgaacgcaac, ceh-60_2, 2, 23, 55
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tctctcaaagatcggtgtga, ceh-60_3, 3, 45, 45
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agctgcttaataagattatc, ceh-60_4, 4, 67, 30
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ttcacttgagacgctcattt, ceh-60_5, 5, 89, 40
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caataaaattcctgtcgacg, ceh-60_6, 6, 111, 40
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ggattcatttcgatgcattt, ceh-60_7, 7, 133, 35
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ttcgtgaagcatccggcgac, ceh-60_8, 8, 155, 60
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tgtcttttctttcaagtagc, ceh-60_9, 9, 177, 35
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aagttcatccgagagcgtaa, ceh-60_10, 10, 199, 45
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catctgtatcatacaacgca, ceh-60_11, 11, 222, 40
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tcctcaatttcggaggattg, ceh-60_12, 12, 244, 45
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gattttcgaattgagcgaga, ceh-60_13, 13, 267, 40
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acctcttccaacggaaatgt, ceh-60_14, 14, 289, 45
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ttcctcgtctttctcagtgt, ceh-60_15, 15, 311, 45
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atttcctttctaagggttgc, ceh-60_16, 16, 333, 40
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atacggttaattccgtccaa, ceh-60_17, 17, 355, 40
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gtttcatgatagtgtcttct, ceh-60_18, 18, 378, 35
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catatccttgtccagttgaa, ceh-60_19, 19, 401, 40
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cgctattagtcagggctttt, ceh-60_20, 20, 423, 45
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cgatgatttctgagtacttc, ceh-60_21, 21, 445, 40
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ttcgtcgatcggtctaaaat, ceh-60_22, 22, 467, 40
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gccgaatgttagagaaatcc, ceh-60_23, 23, 489, 45
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ctcgaaccttttggaaatgc, ceh-60_24, 24, 512, 45
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cgcgaatgttatttttcgaa, ceh-60_25, 25, 534, 35
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agaattttagttgctgcctc, ceh-60_26, 26, 556, 40
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ctcgatctccctccggagaa, ceh-60_27, 27, 578, 60
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ttccggcgcttgcgtccctg, ceh-60_28, 28, 601, 70
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gtccgtcgtgtttttgtcaa, ceh-60_29, 29, 623, 45
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cgtggaaccagttttgcaaa, ceh-60_30, 30, 645, 45
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gggtaaggattttgacgatg, ceh-60_31, 31, 667, 45
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attccgccttttcctggtcg, ceh-60_32, 32, 690, 55
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ttgatattacactgttttgc, ceh-60_33, 33, 712, 30
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ccaattgttaacctgtgaaa, ceh-60_34, 34, 734, 35
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gtccgaattcgttgatttcc, ceh-60_35, 35, 757, 45
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catacgcaaagcttgttgtt, ceh-60_36, 36, 779, 40
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ctctttcacgttcatcttct, ceh-60_37, 37, 801, 40
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gcttcgttagccattgatgc, ceh-60_38, 38, 823, 50
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caaagaattctgaattgctt, ceh-60_39, 39, 845, 30
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aagatgcaaccgtagaactg, ceh-60_40, 40, 867, 45
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cccgatgtgctcatattcga, ceh-60_41, 41, 889, 50
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aaccagcggaatgttaatga, ceh-60_42, 42, 911, 40
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ccattgattgcattgttggg, ceh-60_43, 43, 933, 45
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cctggttgcactgcaggaat, ceh-60_44, 44, 955, 55
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accgtttggattgccaagta, ceh-60_45, 45, 977, 45

Current revision

Progress:

Probes designed: (03-01-2010 - Alexander van Oudenaarden)

Probes coupled:

Probes purified:

Probes tested:

Dyes available:


>F22A3.5 (ceh-60 spliced coding region)

agacacatcacagctctgtgttgttgcgttcacaaccgagtgcatcacaccgatctttgagag

atggataatcttattaagcagctccaaatgagcgtctcaagtgaaaacgtcgacaggaattttattgaaaaatgcatcga aatgaatccctgtcgccggatgcttcacgaagtgctacttgaaagaaaagacattttacgctctcggatgaacttggttg cgttgtatgatacagatgaacaatcctccgaaattgaggaaattctcgctcaattcgaaaatcctacatttccgttggaa gaggtcaacactgagaaagacgaggaatggcaacccttagaaaggaaatatttggacggaattaaccgtataaaagaaga cactatcatgaaacaaattcaactggacaaggatatggaaaaagccctgactaatagcgaagaagtactcagaaatcatc gcgattttagaccgatcgacgaaaaggatttctctaacattcggcaaagcatttccaaaaggttcgagcattcgaaaaat aacattcgcggggaggcagcaactaaaattctagttctccggagggagatcgagcagcagggacgcaagcgccggaactt tgacaaaaacacgacggacattttgcaaaactggttccacgatcatcgtcaaaatccttacccgtccgaccaggaaaagg cggaattagcaaaacagtgtaatatcaaaatttcacaggttaacaattggtttggaaatcaacgaattcggacaaaacaa caagctttgcgtatgcaagaagatgaacgtgaaagagctgcatcaatggctaacgaagcacaagcaattcagaattcttt gaacagttctacggttgcatcttcgtcgaatatgagcacatcggggctcattaacattccgctggttaacccaacaatgc aatcaatggtaattcctgcagtgcaaccaggcctacttggcaatccaaacggtcagttataa



Name of probe set: ceh-60 Probes designed on Mon, 01 Mar 10 13:51:37 -0700 45 probes designed for target of length 1005 (target too short for 48 probes)

Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC

cacagagctgtgatgtgtct, ceh-60_1, 1, 1, 50

cactcggttgtgaacgcaac, ceh-60_2, 2, 23, 55

tctctcaaagatcggtgtga, ceh-60_3, 3, 45, 45

agctgcttaataagattatc, ceh-60_4, 4, 67, 30

ttcacttgagacgctcattt, ceh-60_5, 5, 89, 40

caataaaattcctgtcgacg, ceh-60_6, 6, 111, 40

ggattcatttcgatgcattt, ceh-60_7, 7, 133, 35

ttcgtgaagcatccggcgac, ceh-60_8, 8, 155, 60

tgtcttttctttcaagtagc, ceh-60_9, 9, 177, 35

aagttcatccgagagcgtaa, ceh-60_10, 10, 199, 45

catctgtatcatacaacgca, ceh-60_11, 11, 222, 40

tcctcaatttcggaggattg, ceh-60_12, 12, 244, 45

gattttcgaattgagcgaga, ceh-60_13, 13, 267, 40

acctcttccaacggaaatgt, ceh-60_14, 14, 289, 45

ttcctcgtctttctcagtgt, ceh-60_15, 15, 311, 45

atttcctttctaagggttgc, ceh-60_16, 16, 333, 40

atacggttaattccgtccaa, ceh-60_17, 17, 355, 40

gtttcatgatagtgtcttct, ceh-60_18, 18, 378, 35

catatccttgtccagttgaa, ceh-60_19, 19, 401, 40

cgctattagtcagggctttt, ceh-60_20, 20, 423, 45

cgatgatttctgagtacttc, ceh-60_21, 21, 445, 40

ttcgtcgatcggtctaaaat, ceh-60_22, 22, 467, 40

gccgaatgttagagaaatcc, ceh-60_23, 23, 489, 45

ctcgaaccttttggaaatgc, ceh-60_24, 24, 512, 45

cgcgaatgttatttttcgaa, ceh-60_25, 25, 534, 35

agaattttagttgctgcctc, ceh-60_26, 26, 556, 40

ctcgatctccctccggagaa, ceh-60_27, 27, 578, 60

ttccggcgcttgcgtccctg, ceh-60_28, 28, 601, 70

gtccgtcgtgtttttgtcaa, ceh-60_29, 29, 623, 45

cgtggaaccagttttgcaaa, ceh-60_30, 30, 645, 45

gggtaaggattttgacgatg, ceh-60_31, 31, 667, 45

attccgccttttcctggtcg, ceh-60_32, 32, 690, 55

ttgatattacactgttttgc, ceh-60_33, 33, 712, 30

ccaattgttaacctgtgaaa, ceh-60_34, 34, 734, 35

gtccgaattcgttgatttcc, ceh-60_35, 35, 757, 45

catacgcaaagcttgttgtt, ceh-60_36, 36, 779, 40

ctctttcacgttcatcttct, ceh-60_37, 37, 801, 40

gcttcgttagccattgatgc, ceh-60_38, 38, 823, 50

caaagaattctgaattgctt, ceh-60_39, 39, 845, 30

aagatgcaaccgtagaactg, ceh-60_40, 40, 867, 45

cccgatgtgctcatattcga, ceh-60_41, 41, 889, 50

aaccagcggaatgttaatga, ceh-60_42, 42, 911, 40

ccattgattgcattgttggg, ceh-60_43, 43, 933, 45

cctggttgcactgcaggaat, ceh-60_44, 44, 955, 55

accgtttggattgccaagta, ceh-60_45, 45, 977, 45

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