Van Oudenaarden Lab:T01D3.2
>T01D3.2 (spliced + UTR - 985) gagaaATGGA GACTAATCTT TCTGAAGAAA AACAAAAACC GTCAAAATCA CAAGCCCAGC AGAGAAGACA AATGGAAAAT TATGAATTCT CACAGCTAGC CAATGAACTT CCATTGGCAA GAGCAATTTC TGGACAGCAT ATTGATAAAA CAACGATGGT TAGATTAGCA ACTGCTTATA TCAAACTTCA CAATATATTT GGGCAATCTC AAAGAGCCTA CAGTTCAGCT GACTATTATT ATGGAAGTGA CTCTTTATGG ACTAACAATC ATTTAGATCT ACTAGATGGA TTCTTTGTGA TACTGGACAG GCGCGGAGAT GTACTTTACA TAAGTGAAAC TATTAGTATT TACTTGGGCT TGTCACAAGT TGAAATGACT GGCAACGCGA TGGTTGATTA TATTCATGAA CAGGATATCA ATTGCTTTAA CTCGGCCCTT AACTATTGTG ACCTTAATTG GCCTCAAATG TGCAACGTAC GTGTTAAATC TAGTCTCACA AAGCGAGCAA ACAAGGATGC AGTTCGTGCT TCACCTGGAT ACAAGGTTCT TCGTCTAGAA ATTACAATGG GTCCAAATAC AAATACTAGA ATGATTGCAT GTTATCCAAT GCCTACTCCA GTTCTTTCTA CAGTAACCAT TCCTTCTAAT TCATTTGTTA TCATTACATC CATCGATCTT CATATAACAT TTGCTGATGA AAAAGCTCAT CAGTTGCTTA ATAACCCATT TTACCCAGAT TCAAATATCA AAGGAATGTC ATTATATTCA CTGATTGATA TCTCAGATTC TGAAGTTATA TCAAAAATGC ATTTCGATAT TTTCAACCTG GGCGCTTATA AAACCCCGTA CTATCGAATG ATCTTGAATC AAACAAGTGA AACTTTTTAC GTCGAGTCAA ATATTTTTCG TCATACTTCG ATTTCCTCTA AACAGTTTAA TGACTCGATC ACATTTGTTT CCTCAATTCT ATGAcctcca acttt
Name of probe set: hlh-34 Probes designed on Wed, 03 Mar 10 23:49:08 -0700 40 probes designed for target of length 969
Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC
cttcagaaagattagtctcc, hlh-34_1, 1, 3, 40 ggcttgtgattttgacggtt, hlh-34_2, 2, 32, 45 tttccatttgtcttctctgc, hlh-34_3, 3, 54, 40 ggctagctgtgagaattcat, hlh-34_4, 4, 77, 45 ctcttgccaatggaagttca, hlh-34_5, 5, 99, 45 tcaatatgctgtccagaaat, hlh-34_6, 6, 121, 35 gctaatctaaccatcgttgt, hlh-34_7, 7, 145, 40 gtgaagtttgatataagcag, hlh-34_8, 8, 167, 35 ctttgagattgcccaaatat, hlh-34_9, 9, 190, 35 atagtcagctgaactgtagg, hlh-34_10, 10, 212, 45 gtccataaagagtcacttcc, hlh-34_11, 11, 238, 45 ccatctagtagatctaaatg, hlh-34_12, 12, 265, 35 cctgtccagtatcacaaaga, hlh-34_13, 13, 287, 45 ttatgtaaagtacatctccg, hlh-34_14, 14, 309, 35 gcccaagtaaatactaatag, hlh-34_15, 15, 335, 35 gccagtcatttcaacttgtg, hlh-34_16, 16, 359, 45 gaatataatcaaccatcgcg, hlh-34_17, 17, 381, 40 aagcaattgatatcctgttc, hlh-34_18, 18, 403, 35 acaatagttaagggccgagt, hlh-34_19, 19, 425, 45 acatttgaggccaattaagg, hlh-34_20, 20, 447, 40 gactagatttaacacgtacg, hlh-34_21, 21, 471, 40 tccttgtttgctcgctttgt, hlh-34_22, 22, 493, 45 tccaggtgaagcacgaactg, hlh-34_23, 23, 515, 55 tttctagacgaagaaccttg, hlh-34_24, 24, 537, 40 tttgtatttggacccattgt, hlh-34_25, 25, 559, 35 ggataacatgcaatcattct, hlh-34_26, 26, 583, 35 agaaagaactggagtaggca, hlh-34_27, 27, 605, 45 gaattagaaggaatggttac, hlh-34_28, 28, 628, 35 gaagatcgatggatgtaatg, hlh-34_29, 29, 657, 40 gatgagctttttcatcagca, hlh-34_30, 30, 687, 40 gaatctgggtaaaatgggtt, hlh-34_31, 31, 718, 40 gtgaatataatgacattcct, hlh-34_32, 32, 747, 30 cttcagaatctgagatatca, hlh-34_33, 33, 771, 35 ggttgaaaatatcgaaatgc, hlh-34_34, 34, 804, 35 agtacggggttttataagcg, hlh-34_35, 35, 828, 45 gtttgattcaagatcattcg, hlh-34_36, 36, 850, 35 ctcgacgtaaaaagtttcac, hlh-34_37, 37, 872, 40 gaaatcgaagtatgacgaaa, hlh-34_38, 38, 901, 35 cgagtcattaaactgtttag, hlh-34_39, 39, 923, 35 gaattgaggaaacaaatgtg, hlh-34_40, 40, 945, 35
Input sequence with probe locations:
atggagactaatctttctgaagaaaaacaaaaaccgtcaaaatcacaagcccagcagagaagacaaatgg
cctctgattagaaagacttc ttggcagttttagtgttcgg cgtctcttctgtttacc Probe # 1, 40% GC Probe # 2, 45% GC Probe # 3, 40% GC
aaaattatgaattctcacagctagccaatgaacttccattggcaagagcaatttctggacagcatattga ttt tacttaagagtgtcgatcgg acttgaaggtaaccgttctc taaagacctgtcgtataact
Probe # 4, 45% GC Probe # 5, 45% GC Probe # 6, 35% GC
taaaacaacgatggttagattagcaactgcttatatcaaacttcacaatatatttgggcaatctcaaaga
tgttgctaccaatctaatcg gacgaatatagtttgaagtg tataaacccgttagagtttc Probe # 7, 40% GC Probe # 8, 35% GC Probe # 9, 35% GC
gcctacagttcagctgactattattatggaagtgactctttatggactaacaatcatttagatctactag
ggatgtcaagtcgactgata ccttcactgagaaatacctg gtaaatctagatgatc Probe # 10, 45% GC Probe # 11, 45% GC Probe # 12, 35%
atggattctttgtgatactggacaggcgcggagatgtactttacataagtgaaactattagtatttactt tacc agaaacactatgacctgtcc gcctctacatgaaatgtatt gataatcataaatgaa GC Probe # 13, 45% GC Probe # 14, 35% GC Probe # 15, 35%
gggcttgtcacaagttgaaatgactggcaacgcgatggttgattatattcatgaacaggatatcaattgc cccg gtgttcaactttactgaccg gcgctaccaactaatataag cttgtcctatagttaacg GC Probe # 16, 45% GC Probe # 17, 40% GC Probe # 18, 35% GC
tttaactcggcccttaactattgtgaccttaattggcctcaaatgtgcaacgtacgtgttaaatctagtc aa tgagccgggaattgataaca ggaattaaccggagtttaca gcatgcacaatttagatcag
Probe # 19, 45% GC Probe # 20, 40% GC Probe # 21, 40% GC
tcacaaagcgagcaaacaaggatgcagttcgtgcttcacctggatacaaggttcttcgtctagaaattac
tgtttcgctcgtttgttcct gtcaagcacgaagtggacct gttccaagaagcagatcttt tg Probe # 22, 45% GC Probe # 23, 55% GC Probe # 24, 40% GC Pr
aatgggtccaaatacaaatactagaatgattgcatgttatccaatgcctactccagttctttctacagta ttacccaggtttatgttt tcttactaacgtacaatagg acggatgaggtcaagaaaga cat obe # 25, 35% GC Probe # 26, 35% GC Probe # 27, 45% GC Pro
accattccttctaattcatttgttatcattacatccatcgatcttcatataacatttgctgatgaaaaag tggtaaggaagattaag gtaatgtaggtagctagaag acgactactttttc be # 28, 35% GC Probe # 29, 40% GC Probe # 30, 40
ctcatcagttgcttaataacccattttacccagattcaaatatcaaaggaatgtcattatattcactgat gagtag ttgggtaaaatgggtctaag tccttacagtaatataagtg % GC Probe # 31, 40% GC Probe # 32, 30% GC
tgatatctcagattctgaagttatatcaaaaatgcatttcgatattttcaacctgggcgcttataaaacc actatagagtctaagacttc cgtaaagctataaaagttgg gcgaatattttgg Probe # 33, 35% GC Probe # 34, 35% GC Probe # 35, 4
ccgtactatcgaatgatcttgaatcaaacaagtgaaactttttacgtcgagtcaaatatttttcgtcata ggcatga gcttactagaacttagtttg cactttgaaaaatgcagctc aaagcagtat 5% GC Probe # 36, 35% GC Probe # 37, 40% GC Probe # 38
cttcgatttcctctaaacagtttaatgactcgatcacatttgtttcctcaattctatga gaagctaaag gatttgtcaaattactgagc gtgtaaacaaaggagttaag , 35% GC Probe # 39, 35% GC Probe # 40, 35% GC
