Van Oudenaarden Lab:T01E8.2

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Current revision (21:50, 1 March 2010) (view source)
 
Line 34: Line 34:
Name of probe set: ref-1
Name of probe set: ref-1
-
Probes designed on Mon, 01 Mar 10 14:48:56 -0700
+
Probes designed on Mon, 01 Mar 10 18:49:58 -0700
 +
 
48 probes designed for target of length 1104
48 probes designed for target of length 1104
Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC
Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC
-
ggggtactgatgaggaccat, Probe_1, 1, 1, 55
+
ggggtactgatgaggaccat, ref-1_1, 1, 1, 55
-
tgttatgagcgtaagctggt, Probe_2, 2, 24, 45
+
tgttatgagcgtaagctggt, ref-1_2, 2, 24, 45
-
cgtttcttctcctgtgatgt, Probe_3, 3, 49, 45
+
cgtttcttctcctgtgatgt, ref-1_3, 3, 49, 45
-
ctttgcattaatctcatctc, Probe_4, 4, 71, 35
+
ctttgcattaatctcatctc, ref-1_4, 4, 71, 35
-
tcaatagttgaagctccttg, Probe_5, 5, 93, 40
+
tcaatagttgaagctccttg, ref-1_5, 5, 93, 40
-
ggtcattttctcattatccg, Probe_6, 6, 125, 40
+
ggtcattttctcattatccg, ref-1_6, 6, 125, 40
-
cacggtttaagacatcacct, Probe_7, 7, 147, 45
+
cacggtttaagacatcacct, ref-1_7, 7, 147, 45
-
atgcgactgaccacttcgac, Probe_8, 8, 169, 55
+
atgcgactgaccacttcgac, ref-1_8, 8, 169, 55
-
tggtccaggtgattcggttt, Probe_9, 9, 191, 50
+
tggtccaggtgattcggttt, ref-1_9, 9, 191, 50
-
cctttgcgatttggatttga, Probe_10, 10, 214, 40
+
cctttgcgatttggatttga, ref-1_10, 10, 214, 40
-
tgatcggaatccgtcaaaga, Probe_11, 11, 236, 45
+
tgatcggaatccgtcaaaga, ref-1_11, 11, 236, 45
-
aaggaataagtgagcgactc, Probe_12, 12, 259, 45
+
aaggaataagtgagcgactc, ref-1_12, 12, 259, 45
-
attgacgcccagggatttga, Probe_13, 13, 281, 50
+
attgacgcccagggatttga, ref-1_13, 13, 281, 50
-
caaaatcctgacagacatct, Probe_14, 14, 303, 40
+
caaaatcctgacagacatct, ref-1_14, 14, 303, 40
-
aaaaactgcttggcacgttg, Probe_15, 15, 325, 45
+
aaaaactgcttggcacgttg, ref-1_15, 15, 325, 45
-
caaagatgaacgttctcgat, Probe_16, 16, 347, 40
+
caaagatgaacgttctcgat, ref-1_16, 16, 347, 40
-
attttccagatacagtgctc, Probe_17, 17, 369, 40
+
attttccagatacagtgctc, ref-1_17, 17, 369, 40
-
tccgactcagatcttctctt, Probe_18, 18, 391, 45
+
tccgactcagatcttctctt, ref-1_18, 18, 391, 45
-
gctcattgaagagtggagaa, Probe_19, 19, 413, 45
+
gctcattgaagagtggagaa, ref-1_19, 19, 413, 45
-
gagagcttgactgggatcta, Probe_20, 20, 435, 50
+
gagagcttgactgggatcta, ref-1_20, 20, 435, 50
-
attccagattccgatgtcga, Probe_21, 21, 457, 45
+
attccagattccgatgtcga, ref-1_21, 21, 457, 45
-
cacttcctttctatcaattg, Probe_22, 22, 479, 35
+
cacttcctttctatcaattg, ref-1_22, 22, 479, 35
-
ctcttctatcttgctctcta, Probe_23, 23, 507, 40
+
ctcttctatcttgctctcta, ref-1_23, 23, 507, 40
-
atcaaaagcttctccctgac, Probe_24, 24, 530, 45
+
atcaaaagcttctccctgac, ref-1_24, 24, 530, 45
-
ctcaatgataaagttcttca, Probe_25, 25, 554, 30
+
ctcaatgataaagttcttca, ref-1_25, 25, 554, 30
-
gatgagatgtcatgagcttg, Probe_26, 26, 576, 45
+
gatgagatgtcatgagcttg, ref-1_26, 26, 576, 45
-
aagcgttgcattttttcgac, Probe_27, 27, 598, 40
+
aagcgttgcattttttcgac, ref-1_27, 27, 598, 40
-
caattatgatatccagggtg, Probe_28, 28, 621, 40
+
caattatgatatccagggtg, ref-1_28, 28, 621, 40
-
gtgcttcttgttttgaatgt, Probe_29, 29, 644, 35
+
gtgcttcttgttttgaatgt, ref-1_29, 29, 644, 35
-
ggtcgcttcgactaacaaaa, Probe_30, 30, 666, 45
+
ggtcgcttcgactaacaaaa, ref-1_30, 30, 666, 45
-
tgtgcatagagtgactgctc, Probe_31, 31, 688, 50
+
tgtgcatagagtgactgctc, ref-1_31, 31, 688, 50
-
cttctttccctctgcaattg, Probe_32, 32, 710, 45
+
cttctttccctctgcaattg, ref-1_32, 32, 710, 45
-
ggaaggcaatgttttttgca, Probe_33, 33, 732, 40
+
ggaaggcaatgttttttgca, ref-1_33, 33, 732, 40
-
tgacggtcgctcttgaaaaa, Probe_34, 34, 754, 45
+
tgacggtcgctcttgaaaaa, ref-1_34, 34, 754, 45
-
acttttcaaggtctgcgcat, Probe_35, 35, 786, 45
+
acttttcaaggtctgcgcat, ref-1_35, 35, 786, 45
-
ggctttggtgataaggagaa, Probe_36, 36, 814, 45
+
ggctttggtgataaggagaa, ref-1_36, 36, 814, 45
-
catggatgggaatccaaaga, Probe_37, 37, 836, 45
+
catggatgggaatccaaaga, ref-1_37, 37, 836, 45
-
atggtggtggaattgggatg, Probe_38, 38, 858, 50
+
atggtggtggaattgggatg, ref-1_38, 38, 858, 50
-
cggaatgggaagattgggaa, Probe_39, 39, 880, 50
+
cggaatgggaagattgggaa, ref-1_39, 39, 880, 50
-
cattgggaagaatgggaatg, Probe_40, 40, 902, 45
+
cattgggaagaatgggaatg, ref-1_40, 40, 902, 45
-
ttgttgccattggtgccatt, Probe_41, 41, 924, 45
+
ttgttgccattggtgccatt, ref-1_41, 41, 924, 45
-
ggagactgttgagattttgg, Probe_42, 42, 946, 45
+
ggagactgttgagattttgg, ref-1_42, 42, 946, 45
-
ggtggagaatccagtgaata, Probe_43, 43, 970, 45
+
ggtggagaatccagtgaata, ref-1_43, 43, 970, 45
-
cgaagaagtatcggatgatg, Probe_44, 44, 992, 45
+
cgaagaagtatcggatgatg, ref-1_44, 44, 992, 45
-
gtggatggtgtttcgattga, Probe_45, 45, 1015, 45
+
gtggatggtgtttcgattga, ref-1_45, 45, 1015, 45
-
ggaattcgagttctcatttg, Probe_46, 46, 1037, 40
+
ggaattcgagttctcatttg, ref-1_46, 46, 1037, 40
-
ctctttcttgaagctttagg, Probe_47, 47, 1060, 40
+
ctctttcttgaagctttagg, ref-1_47, 47, 1060, 40
-
ttcccatggtctgaacagct, Probe_48, 48, 1082, 50
+
ttcccatggtctgaacagct, ref-1_48, 48, 1082, 50

Current revision

Progress:

Probes designed: (03-01-2010 - Jialing Li)

Probes coupled:

Probes purified:

Probes tested:

Dyes available:


>T01E8.2 (ref-1 spliced coding region)

atggtcctcatcagtaccccaccaccagcttacgctcataacagaaaaacatcacaggagaagaaacgacgagatgagat taatgcaaagatcaaggagcttcaactattgattcaaaatgaatcggataatgagaaaatgacccaaggtgatgtcttaa accgtgcagtcgaagtggtcagtcgcatggaaaccgaatcacctggaccatcatcaaatccaaatcgcaaaggattcttt gacggattccgatcaatcgagtcgctcacttattccttcatcaaatccctgggcgtcaattcagatgtctgtcaggattt tgtgcaacgtgccaagcagtttttcgatcgagaacgttcatctttgctgagcactgtatctggaaaatcaaagagaagat ctgagtcggaaattctccactcttcaatgagctatagatcccagtcaagctctccatcgacatcggaatctggaatcaca attgatagaaaggaagtgaaaaagaatagagagcaagatagaagagatcgtcagggagaagcttttgatgctttgaagaa ctttatcattgagaacaagctcatgacatctcatcaagtcgaaaaaatgcaacgcttgaacaccctggatatcataattg cctacattcaaaacaagaagcacaattttgttagtcgaagcgaccaggagcagtcactctatgcacatgcaattgcagag ggaaagaagactgcaaaaaacattgccttccaatttttcaagagcgaccgtcacctcgttgtccgatgcgcagaccttga aaagttcttcgaattctccttatcaccaaagccactctttggattcccatccatgcccatcccaattccaccaccatcat tcccaatcttcccattccggccattcccattcttcccaatgccaatggcaccaatggcaacaagtccaaaatctcaacag tctccatcatattcactggattctccaccaccatcatccgatacttcttcgtcatcaatcgaaacaccatccactccaaa tgagaactcgaattccaatcctaaagcttcaagaaagagtaagctgttcagaccatgggaataa


Name of probe set: ref-1

Probes designed on Mon, 01 Mar 10 18:49:58 -0700

48 probes designed for target of length 1104

Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC

ggggtactgatgaggaccat, ref-1_1, 1, 1, 55

tgttatgagcgtaagctggt, ref-1_2, 2, 24, 45

cgtttcttctcctgtgatgt, ref-1_3, 3, 49, 45

ctttgcattaatctcatctc, ref-1_4, 4, 71, 35

tcaatagttgaagctccttg, ref-1_5, 5, 93, 40

ggtcattttctcattatccg, ref-1_6, 6, 125, 40

cacggtttaagacatcacct, ref-1_7, 7, 147, 45

atgcgactgaccacttcgac, ref-1_8, 8, 169, 55

tggtccaggtgattcggttt, ref-1_9, 9, 191, 50

cctttgcgatttggatttga, ref-1_10, 10, 214, 40

tgatcggaatccgtcaaaga, ref-1_11, 11, 236, 45

aaggaataagtgagcgactc, ref-1_12, 12, 259, 45

attgacgcccagggatttga, ref-1_13, 13, 281, 50

caaaatcctgacagacatct, ref-1_14, 14, 303, 40

aaaaactgcttggcacgttg, ref-1_15, 15, 325, 45

caaagatgaacgttctcgat, ref-1_16, 16, 347, 40

attttccagatacagtgctc, ref-1_17, 17, 369, 40

tccgactcagatcttctctt, ref-1_18, 18, 391, 45

gctcattgaagagtggagaa, ref-1_19, 19, 413, 45

gagagcttgactgggatcta, ref-1_20, 20, 435, 50

attccagattccgatgtcga, ref-1_21, 21, 457, 45

cacttcctttctatcaattg, ref-1_22, 22, 479, 35

ctcttctatcttgctctcta, ref-1_23, 23, 507, 40

atcaaaagcttctccctgac, ref-1_24, 24, 530, 45

ctcaatgataaagttcttca, ref-1_25, 25, 554, 30

gatgagatgtcatgagcttg, ref-1_26, 26, 576, 45

aagcgttgcattttttcgac, ref-1_27, 27, 598, 40

caattatgatatccagggtg, ref-1_28, 28, 621, 40

gtgcttcttgttttgaatgt, ref-1_29, 29, 644, 35

ggtcgcttcgactaacaaaa, ref-1_30, 30, 666, 45

tgtgcatagagtgactgctc, ref-1_31, 31, 688, 50

cttctttccctctgcaattg, ref-1_32, 32, 710, 45

ggaaggcaatgttttttgca, ref-1_33, 33, 732, 40

tgacggtcgctcttgaaaaa, ref-1_34, 34, 754, 45

acttttcaaggtctgcgcat, ref-1_35, 35, 786, 45

ggctttggtgataaggagaa, ref-1_36, 36, 814, 45

catggatgggaatccaaaga, ref-1_37, 37, 836, 45

atggtggtggaattgggatg, ref-1_38, 38, 858, 50

cggaatgggaagattgggaa, ref-1_39, 39, 880, 50

cattgggaagaatgggaatg, ref-1_40, 40, 902, 45

ttgttgccattggtgccatt, ref-1_41, 41, 924, 45

ggagactgttgagattttgg, ref-1_42, 42, 946, 45

ggtggagaatccagtgaata, ref-1_43, 43, 970, 45

cgaagaagtatcggatgatg, ref-1_44, 44, 992, 45

gtggatggtgtttcgattga, ref-1_45, 45, 1015, 45

ggaattcgagttctcatttg, ref-1_46, 46, 1037, 40

ctctttcttgaagctttagg, ref-1_47, 47, 1060, 40

ttcccatggtctgaacagct, ref-1_48, 48, 1082, 50

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