Van Oudenaarden Lab:W02C12.3: Difference between revisions
(New page: ------------------------------------------------ >W02C12.3a (spliced + UTR - 1558) cagcaattca tacacaattg atcgtccccg tccatataac cgctgaaaaa aaggaaaaaa aATGGCCGAT GACGACGACC GCGTTGACAT TT...) |
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Revision as of 23:14, 3 March 2010
>W02C12.3a (spliced + UTR - 1558) cagcaattca tacacaattg atcgtccccg tccatataac cgctgaaaaa aaggaaaaaa aATGGCCGAT GACGACGACC GCGTTGACAT TTCCAACGAG ACGCTTGCCT CTTTGGTGGG TCGACCGATG AGCTCGTCGG GCAATCGGAT TCGAGCCACC CCATCCAGAC GGCGGCTGGT TTCTGCGCCG ACGAACCCGA GGTGGTCACC ATCCGCAGAC GATCGGCGTC TTTTTTCATA TGTCTCGTCG ATGCGGTCGG ATAGTAACAG TAGCAGCAAG TCACCATCTC CACCGTCGGC CAAAATGTCT AAAAGGCCGA ATGGCCCGTA CTCTGTTAGA AAATATCAGC CCCGCGGATC CCCACCCAGT GACGGCAGCG ACAAAATTCA CAGATTCGGA GAGAGCCCAA CGCCCGGTGG TGTCGGTGGT GTATTTGGCA CAGAGCTTGA CGACCTGATC ATTGATGAGC TCATGGGCAT GGAAGATGAT CAGCGAATGC GTCCAGGAGC GACACGACCG ATGACGATTG GTGGAGAAAA AACCATGTCG ATGGCAAGAC CGATTCCAGG CGCCAGCTCC CGGGCTGGCT CAGGACACTC TGGAAGCCCG ATCACAATTC CAAACGCAAT GTCGAACAAT TTCAGACAGG TCGTCTCAAG TTCGGCGCCG ACGAGTTCGA TCGACATTGA GAAGATGATT GGCGCCGTGT CGAACGGCGG TGGGAATAGT GGCGGTGATA ATGATCCGGA GGACTATTAC CGCGACCGCA GGAAGAAGGA CATTCACAAT ATGATTGAAC GCCGACGAAG ATATAATATA AATGACAGGA TTAAGGAGCT CGGACAAATG TTGCCGAAGA ACACATCAGA AGACATGAAA CTCAACAAAG GAACAATTCT AAAAGCCTCG TGTGACTACA TCCGAGTGCT CCAAAAAGAT CGCGAGCAAG CGATGAAAAC CCAACAGCAG CAGAAATCGC TGGAATCGAC GGCTCACAAG TACGCCGACC GGGTGAAAGA GCTCGAGGAG ATGCTCGCCA GGCAGGGAGT GCAGGTCCCG CCATCGCATC TTCCACCGAT CCCGAAAGTG ATCGAGCGAC CGATTAAGCA GGAAATCGAC GAGTCGCCAC CAAATCACAC GCCGACCGGA TCGTTTGTGT CCTCATCCGG CTTCCTATCC GAGGTGACAA ACAACACCGC CGCCATGCAG ATCACATCGC CGAACGACTC GCGCCCGAAC AACTTCATGA ACAACTCGGC GCCGTCGGAC AGCTTCTTCT CGGTCGGATC GGCTAGCCCG CCAGATTATA GGACGAGCAG CGGCACCGCG TCGTGGAAGC TGCCCGGATC GAACGCCTTC TCCGACCTCA TGATGGACGA TCTCAACCCA ATGATGAACG GCGATCCACT CATCTCATCG GCCGGCGCTC ATCCATCACC ACACTTCCAC AGCTCACAAA TGTCACCAGA CATTCATTGG GACGCCTCTG GCTTCTCCCC AGACCCGATC AACACCCAAC AGTCAAACTC GGGTCATTAT CACATGGACT TTTCGTAA
Name of probe set: hlh-25 Probes designed on Wed, 03 Mar 10 23:14:11 -0700 44 probes designed for target of length 1558
Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC
ggacgatcaattgtgtatga, hlh-25_1, 1, 8, 40 ttttcagcggttatatggac, hlh-25_2, 2, 30, 40 gtcgtcatcggccatttttt, hlh-25_3, 3, 57, 45 tctcgttggaaatgtcaacg, hlh-25_4, 4, 82, 45 aaaaagacgccgatcgtctg, hlh-25_5, 5, 216, 50 accgcatcgacgagacatat, hlh-25_6, 6, 238, 50 gacttgctgctactgttact, hlh-25_7, 7, 263, 45 tttagacattttggccgacg, hlh-25_8, 8, 294, 45 ttttctaacagagtacgggc, hlh-25_9, 9, 324, 45 tctgtgaattttgtcgctgc, hlh-25_10, 10, 375, 45 aagctctgtgccaaatacac, hlh-25_11, 11, 429, 45 agctcatcaatgatcaggtc, hlh-25_12, 12, 452, 45 ttcgctgatcatcttccatg, hlh-25_13, 13, 478, 45 tttctccaccaatcgtcatc, hlh-25_14, 14, 520, 45 atcggtcttgccatcgacat, hlh-25_15, 15, 545, 50 attgtgatcgggcttccaga, hlh-25_16, 16, 599, 50 attgttcgacattgcgtttg, hlh-25_17, 17, 621, 40 aacttgagacgacctgtctg, hlh-25_18, 18, 643, 50 tcaatgtcgatcgaactcgt, hlh-25_19, 19, 671, 45 attatcaccgccactattcc, hlh-25_20, 20, 723, 45 tgtgaatgtccttcttcctg, hlh-25_21, 21, 769, 45 tatcttcgtcggcgttcaat, hlh-25_22, 22, 794, 45 gagctccttaatcctgtcat, hlh-25_23, 23, 822, 45 gtgttcttcggcaacatttg, hlh-25_24, 24, 845, 45 gttgagtttcatgtcttctg, hlh-25_25, 25, 867, 40 gaggcttttagaattgttcc, hlh-25_26, 26, 890, 40 ttttggagcactcggatgta, hlh-25_27, 27, 917, 45 tttcatcgcttgctcgcgat, hlh-25_28, 28, 939, 50 agcgatttctgctgctgttg, hlh-25_29, 29, 962, 50 gtacttgtgagccgtcgatt, hlh-25_30, 30, 984, 50 atctcctcgagctctttcac, hlh-25_31, 31, 1013, 50 tttcgggatcggtggaagat, hlh-25_32, 32, 1068, 50 atttcctgcttaatcggtcg, hlh-25_33, 33, 1097, 45 atgaggacacaaacgatccg, hlh-25_34, 34, 1147, 50 tttgtcacctcggataggaa, hlh-25_35, 35, 1172, 45 ttcggcgatgtgatctgcat, hlh-25_36, 36, 1205, 50 tgttcatgaagttgttcggg, hlh-25_37, 37, 1234, 45 tcctataatctggcgggcta, hlh-25_38, 38, 1294, 50 tccatcatgaggtcggagaa, hlh-25_39, 39, 1358, 50 cgttcatcattgggttgaga, hlh-25_40, 40, 1381, 45 tttgtgagctgtggaagtgt, hlh-25_41, 41, 1441, 45 tcccaatgaatgtctggtga, hlh-25_42, 42, 1463, 45 ttgactgttgggtgttgatc, hlh-25_43, 43, 1507, 45 tccatgtgataatgacccga, hlh-25_44, 44, 1529, 45
Input sequence with probe locations:
cagcaattcatacacaattgatcgtccccgtccatataaccgctgaaaaaaaggaaaaaaaatggccgat
agtatgtgttaactagcagg caggtatattggcgactttt ttttttaccggcta
Probe # 1, 40% GC Probe # 2, 40% GC Probe # 3, 45%
gacgacgaccgcgttgacatttccaacgagacgcttgcctctttggtgggtcgaccgatgagctcgtcgg ctgctg gcaactgtaaaggttgctct
GC Probe # 4, 45% GC
gcaatcggattcgagccaccccatccagacggcggctggtttctgcgccgacgaacccgaggtggtcacc
atccgcagacgatcggcgtcttttttcatatgtctcgtcgatgcggtcggatagtaacagtagcagcaag
gtctgctagccgcagaaaaa tatacagagcagctacgcca tcattgtcatcgtcgttc
Probe # 5, 50% GC Probe # 6, 50% GC Probe # 7, 45% GC
tcaccatctccaccgtcggccaaaatgtctaaaaggccgaatggcccgtactctgttagaaaatatcagc ag gcagccggttttacagattt cgggcatgagacaatctttt
Probe # 8, 45% GC Probe # 9, 45% GC
cccgcggatccccacccagtgacggcagcgacaaaattcacagattcggagagagcccaacgcccggtgg
cgtcgctgttttaagtgtct
Probe # 10, 45% GC
tgtcggtggtgtatttggcacagagcttgacgacctgatcattgatgagctcatgggcatggaagatgat
cacataaaccgtgtctcgaa ctggactagtaactactcga gtaccttctacta
Probe # 11, 45% GC Probe # 12, 45% GC Probe # 13, 4
cagcgaatgcgtccaggagcgacacgaccgatgacgattggtggagaaaaaaccatgtcgatggcaagac gtcgctt ctactgctaaccacctcttt tacagctaccgttctg 5% GC Probe # 14, 45% GC Probe # 15, 50%
cgattccaggcgccagctcccgggctggctcaggacactctggaagcccgatcacaattccaaacgcaat gcta agaccttcgggctagtgtta gtttgcgtta GC Probe # 16, 50% GC Probe # 17
gtcgaacaatttcagacaggtcgtctcaagttcggcgccgacgagttcgatcgacattgagaagatgatt cagcttgtta gtctgtccagcagagttcaa tgctcaagctagctgtaact , 40% GC Probe # 18, 50% GC Probe # 19, 45% GC
ggcgccgtgtcgaacggcggtgggaatagtggcggtgataatgatccggaggactattaccgcgaccgca
ccttatcaccgccactatta gt
Probe # 20, 45% GC Pr
ggaagaaggacattcacaatatgattgaacgccgacgaagatataatataaatgacaggattaaggagct ccttcttcctgtaagtgt taacttgcggctgcttctat tactgtcctaattcctcga obe # 21, 45% GC Probe # 22, 45% GC Probe # 23, 45% GC
cggacaaatgttgccgaagaacacatcagaagacatgaaactcaacaaaggaacaattctaaaagcctcg g gtttacaacggcttcttgtg gtcttctgtactttgagttg ccttgttaagattttcggag
Probe # 24, 45% GC Probe # 25, 40% GC Probe # 26, 40% GC
tgtgactacatccgagtgctccaaaaagatcgcgagcaagcgatgaaaacccaacagcagcagaaatcgc
atgtaggctcacgaggtttt tagcgctcgttcgctacttt gttgtcgtcgtctttagcg
Probe # 27, 45% GC Probe # 28, 50% GC Probe # 29, 50% GC
tggaatcgacggctcacaagtacgccgaccgggtgaaagagctcgaggagatgctcgccaggcagggagt a ttagctgccgagtgttcatg cactttctcgagctcctcta
Probe # 30, 50% GC Probe # 31, 50% GC
gcaggtcccgccatcgcatcttccaccgatcccgaaagtgatcgagcgaccgattaagcaggaaatcgac
tagaaggtggctagggcttt gctggctaattcgtccttta
Probe # 32, 50% GC Probe # 33, 45% GC
gagtcgccaccaaatcacacgccgaccggatcgtttgtgtcctcatccggcttcctatccgaggtgacaa
gcctagcaaacacaggagta aaggataggctccactgtt
Probe # 34, 50% GC Probe # 35, 45% GC
acaacaccgccgccatgcagatcacatcgccgaacgactcgcgcccgaacaacttcatgaacaactcggc t tacgtctagtgtagcggctt gggcttgttgaagtacttgt
Probe # 36, 50% GC Probe # 37, 45% GC
gccgtcggacagcttcttctcggtcggatcggctagcccgccagattataggacgagcagcggcaccgcg
atcgggcggtctaatatcct
Probe # 38, 50% GC
tcgtggaagctgcccggatcgaacgccttctccgacctcatgatggacgatctcaacccaatgatgaacg
aagaggctggagtactacct agagttgggttactacttgc
Probe # 39, 50% GC Probe # 40, 45% GC
gcgatccactcatctcatcggccggcgctcatccatcaccacacttccacagctcacaaatgtcaccaga
tgtgaaggtgtcgagtgttt agtggtct
Probe # 41, 45% GC Probe #
cattcattgggacgcctctggcttctccccagacccgatcaacacccaacagtcaaactcgggtcattat gtaagtaaccct ctagttgtgggttgtcagtt agcccagtaata 42, 45% GC Probe # 43, 45% GC Probe # 44,
cacatggacttttcgtaa gtgtacct 45% GC
