Vectors: Difference between revisions
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==General vector information== | ==General vector information== | ||
===Stringent vs. relaxed replication=== | |||
Plasmid replication control is usually controlled by balancing the levels of a positive and a negative regulator of replication. For some plasmids (pMB1/colE1 replicons) the positive regulator is an RNA and in others (e.g. pSC101) it is a protein. Plasmids with a protein positive regulator will not replicate in the abscence of protein production - ''stringent control'' (although not the same as the stringent response due to a shortage of loaded tRNAs). Plasmids with an RNA positive regulator will continue to replicate in the abscence of protein production. This is termed ''relaxed control''. High yields of plasmid may be obtained by halting protein production (via chloroamphenicol) when the culture reaches a high density and then continuing incubation for a number of hours. This might be of practical relevance when prepping the 1 and 3 series of Synthetic Biology plasmids.--[[User:Bcanton|BC]] 19:05, 3 Sep 2005 (EDT) | Plasmid replication control is usually controlled by balancing the levels of a positive and a negative regulator of replication. For some plasmids (pMB1/colE1 replicons) the positive regulator is an RNA and in others (e.g. pSC101) it is a protein. Plasmids with a protein positive regulator will not replicate in the abscence of protein production - ''stringent control'' (although not the same as the stringent response due to a shortage of loaded tRNAs). Plasmids with an RNA positive regulator will continue to replicate in the abscence of protein production. This is termed ''relaxed control''. High yields of plasmid may be obtained by halting protein production (via chloroamphenicol) when the culture reaches a high density and then continuing incubation for a number of hours. This might be of practical relevance when prepping the 1 and 3 series of Synthetic Biology plasmids.--[[User:Bcanton|BC]] 19:05, 3 Sep 2005 (EDT) | ||
'''Replicon Compatibility | ==''Escherichia coli''== | ||
[[Vector nomenclature | Nomenclature]] | |||
[[Available vectors | Available]] | |||
[[Vectors to be constructed | To be constructed]] | |||
[[BioBrick Parts for Plasmid Engineering]] | |||
[[Bacterial artificial chromosomes]] | |||
pSCANS [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=Nucleotide&cmd=Retrieve&list_uids=6689911&dopt=GenBank genbank] [http://genome.bnl.gov/Vectors/pscans.php vector info] [http://www.genome.bnl.gov/Protocols/pscans.shtml cookbook] | |||
===Replicon Compatibility=== | |||
''The following are groups of replicons that '''can''' be used with the bold replicon in the one cell''. | ''The following are groups of replicons that '''can''' be used with the bold replicon in the one cell''. | ||
*'''colE1''' - p15A,R6K, and F | *'''colE1''' - p15A,R6K, and F | ||
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*?? | *?? | ||
==Genbank entries | ===Genbank entries=== | ||
''Note: searching for cloning vector <insert vector name> when looking for vector sequences in NCBI Entrez Nucleotide search. It helps to cut down on the number of hits.'' | ''Note: searching for cloning vector <insert vector name> when looking for vector sequences in NCBI Entrez Nucleotide search. It helps to cut down on the number of hits.'' | ||
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*[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=Nucleotide&cmd=Retrieve&list_uids=6689911&dopt=GenBank pSCANS] | *[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=Nucleotide&cmd=Retrieve&list_uids=6689911&dopt=GenBank pSCANS] | ||
== | ==Yeast== | ||
[[Yeast_vectors|Nomenclature and types]] | [[Yeast_vectors|Nomenclature and types]] | ||
Revision as of 16:07, 7 November 2005
This page contains various information relating to vectors used in the Endy lab and other Synthetic Biology labs.
General vector information
Stringent vs. relaxed replication
Plasmid replication control is usually controlled by balancing the levels of a positive and a negative regulator of replication. For some plasmids (pMB1/colE1 replicons) the positive regulator is an RNA and in others (e.g. pSC101) it is a protein. Plasmids with a protein positive regulator will not replicate in the abscence of protein production - stringent control (although not the same as the stringent response due to a shortage of loaded tRNAs). Plasmids with an RNA positive regulator will continue to replicate in the abscence of protein production. This is termed relaxed control. High yields of plasmid may be obtained by halting protein production (via chloroamphenicol) when the culture reaches a high density and then continuing incubation for a number of hours. This might be of practical relevance when prepping the 1 and 3 series of Synthetic Biology plasmids.--BC 19:05, 3 Sep 2005 (EDT)
Escherichia coli
BioBrick Parts for Plasmid Engineering
Bacterial artificial chromosomes
pSCANS genbank vector info cookbook
Replicon Compatibility
The following are groups of replicons that can be used with the bold replicon in the one cell.
- colE1 - p15A,R6K, and F
- pMB1 - p15A,R6K, and F
- ??
Genbank entries
Note: searching for cloning vector <insert vector name> when looking for vector sequences in NCBI Entrez Nucleotide search. It helps to cut down on the number of hits.
