WFYellow(F08) Research Proposal

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(Allen and Jess's 20.109 Module 3 Research Proposal Resource Page)
m (Allen and Jess's 20.109 Module 3 Research Proposal Resource Page)
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==[[User:Allen Lin | Allen]] and [[User:Jessho | Jess's]] 20.109 Module 3 Research Proposal Resource Page==
==[[User:Allen Lin | Allen]] and [[User:Jessho | Jess's]] 20.109 Module 3 Research Proposal Resource Page==
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<b>Idea(s):</b>  
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<b>Idea(s):</b>
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Identify proteins associated with extracellularlly-presented Hsp90 (in particular HER-2/ErbB2), which is known to be over-expressed in proliferating cancer cells.
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##Identify proteins associated with extracellularlly-presented Hsp90 (in particular HER-2/ErbB2), which is known to be over-expressed in proliferating cancer cells.
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##Identify the membrane targeting sequence of Hsp90, produce interfering RNA, and observe phenotypic changes.
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High membrane-bound Hsp90 expression also correlates with the rounded morphology of proliferative cancer.  One potential research idea is to study the induction of flat vs. rounded morphology pathways and observe whether or not return of flattened morphology reduces cell proliferation, metastasis, and tissue invasion.
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##High membrane-bound Hsp90 expression also correlates with the rounded morphology of proliferative cancer.  One potential research idea is to study the induction of flat vs. rounded morphology pathways and observe whether or not return of flattened morphology reduces cell proliferation, metastasis, and tissue invasion.
<b>Links to papers</b>
<b>Links to papers</b>
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<b>Methods</b>
<b>Methods</b>
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<br>cell fractionstion
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<br>cell fractionation
<br>TAP (or other)-tagging
<br>TAP (or other)-tagging
 +
<br>immunofluorescence

Revision as of 14:01, 19 November 2008

Allen and Jess's 20.109 Module 3 Research Proposal Resource Page

Idea(s):

    1. Identify proteins associated with extracellularlly-presented Hsp90 (in particular HER-2/ErbB2), which is known to be over-expressed in proliferating cancer cells.
    2. Identify the membrane targeting sequence of Hsp90, produce interfering RNA, and observe phenotypic changes.
    3. High membrane-bound Hsp90 expression also correlates with the rounded morphology of proliferative cancer. One potential research idea is to study the induction of flat vs. rounded morphology pathways and observe whether or not return of flattened morphology reduces cell proliferation, metastasis, and tissue invasion.

Links to papers

  1. Cid C, Regidor I, Poveda PD, and Alcazar A. . pmid:18800240. PubMed HubMed [cid08]
  2. Sidera K, Gaitanou M, Stellas D, Matsas R, and Patsavoudi E. . pmid:18056992. PubMed HubMed [sidera-jcb-2008]
  3. Eustace BK, Sakurai T, Stewart JK, Yimlamai D, Unger C, Zehetmeier C, Lain B, Torella C, Henning SW, Beste G, Scroggins BT, Neckers L, Ilag LL, and Jay DG. . pmid:15146192. PubMed HubMed [eustace04]
  4. Sidera K and Patsavoudi E. . pmid:18469526. PubMed HubMed [sidera08]
  5. Eustace BK and Jay DG. . pmid:15326368. PubMed HubMed [eustace04b]
  6. Tsutsumi S and Neckers L. . pmid:17645779. PubMed HubMed [tsutsumi07]
All Medline abstracts: PubMed HubMed

Methods
cell fractionation
TAP (or other)-tagging
immunofluorescence

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