WRB100806-Construction of pSB1AK3-J23120
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(Difference between revisions)
| Line 1: | Line 1: | ||
| - | [OriT]-[pTet][Lock3][RFP][TT] | + | [OriT]-[pTet][Lock3][RFP][TT] <br> |
| - | digest pSB1AK3-J23039 (EcoR1, Spe) | + | digest pSB1AK3-J23039 (EcoR1, Spe) <br> |
| - | digest pSB1C3-J23077 (EcoR1, Spe) | + | digest pSB1C3-J23077 (EcoR1, Spe) <br> |
| - | digest pSB1A2-J01003 (Xba, Pst) | + | digest pSB1A2-J01003 (Xba, Pst) <br> |
| - | ligate, product is pSB1AK3-J23120 | + | ligate, product is pSB1AK3-J23120 <br> |
Revision as of 16:06, 8 October 2006
[OriT]-[pTet][Lock3][RFP][TT]
digest pSB1AK3-J23039 (EcoR1, Spe)
digest pSB1C3-J23077 (EcoR1, Spe)
digest pSB1A2-J01003 (Xba, Pst)
ligate, product is pSB1AK3-J23120
