WRB100806-Construction of pSB1AK3-J23120

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[OriT]-[pTet][Lock3][RFP][TT]
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[OriT]-[pTet][Lock3][RFP][TT] <br>
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digest pSB1AK3-J23039 (EcoR1, Spe)
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digest pSB1AK3-J23039 (EcoR1, Spe) <br>
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digest pSB1C3-J23077 (EcoR1, Spe)
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digest pSB1C3-J23077 (EcoR1, Spe) <br>
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digest pSB1A2-J01003 (Xba, Pst)
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digest pSB1A2-J01003 (Xba, Pst) <br>
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ligate, product is pSB1AK3-J23120
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ligate, product is pSB1AK3-J23120 <br>

Revision as of 16:06, 8 October 2006

[OriT]-[pTet][Lock3][RFP][TT]
digest pSB1AK3-J23039 (EcoR1, Spe)
digest pSB1C3-J23077 (EcoR1, Spe)
digest pSB1A2-J01003 (Xba, Pst)
ligate, product is pSB1AK3-J23120

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