Waldminghaus:Back Door/StrainCollection - Revision history

Torsten Waldminghaus: /* Labelling */

2013-01-07T14:42:37Z

Labelling

←Older revision		Revision as of 14:42, 7 January 2013
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	* '''Plasmid''' - If it is a plasmid constructed in our lab the name should be according to our [[Waldminghaus:Back Door/Plasmid \|plasmid list]].		* '''Plasmid''' - If it is a plasmid constructed in our lab the name should be according to our [[Waldminghaus:Back Door/Plasmid \|plasmid list]].
	* '''Relevant Genotype''' - For example pyrB::Tn5, proAB::GATC-cluster, ΔseqA,...		* '''Relevant Genotype''' - For example pyrB::Tn5, proAB::GATC-cluster, ΔseqA,...
		+	* '''Date''' - The date when the strain was stored as glycerole culture. Make sure one can find the details of the strain construction starting at the respective date in your labbook.

	==Strain list==		==Strain list==

	As much information as possible should be stored in the strainlist which can be found in the transfer folder on the SYNMIKRO-server.''' The list and the collection should always be consistent and updated!''' Please also keep a version of the strain list in your private folder.		As much information as possible should be stored in the strainlist which can be found in the transfer folder on the SYNMIKRO-server.''' The list and the collection should always be consistent and updated!''' Please also keep a version of the strain list in your private folder.

Torsten Waldminghaus: /* Strain list */

2013-01-07T13:47:36Z

Strain list

←Older revision		Revision as of 13:47, 7 January 2013
Line 22:		Line 22:
	==Strain list==		==Strain list==

-	As much information as possible should be stored in the ~~[[Waldminghaus:Back Door/Strains\|strain list]]~~.''' The list and the collection should always be consistent and updated!'''	+	As much information as possible should be stored in the strainlist which can be found in the transfer folder on the SYNMIKRO-server.''' The list and the collection should always be consistent and updated!''' Please also keep a version of the strain list in your private folder.

Torsten Waldminghaus: /* General */

2013-01-07T13:45:11Z

General

←Older revision		Revision as of 13:45, 7 January 2013
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	==General==		==General==

-	The strain collection consists of all strains which are constructed in the group or that we get from somewhere. All strains are stored as [[Waldminghaus:Back Door/glycerol culture\|glycerol cultures]] in two copies, one at -80 and one at -20°C.	+	The strain collection consists of all strains which are constructed in the group or that we get from somewhere. All strains are stored as [[Waldminghaus:Back Door/glycerol culture\|glycerol cultures]] in two copies, one at -80 (3. Floor; AG Waldminghaus Freezer) and one at -20°C (Freezer I, Waldminghaus lab).

	==Labelling==		==Labelling==

Torsten Waldminghaus at 12:33, 27 June 2012

2012-06-27T12:33:27Z

←Older revision		Revision as of 12:33, 27 June 2012
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	==General==		==General==

-	The strain collection consists of all strains which are constructed in the group or that we get from somewhere. All strains are stored as [[~~Waldminghau~~:Back Door/glycerol culture\|glycerol cultures]] in two copies, one at -80 and one at -20°C.	+	The strain collection consists of all strains which are constructed in the group or that we get from somewhere. All strains are stored as [[Waldminghaus:Back Door/glycerol culture\|glycerol cultures]] in two copies, one at -80 and one at -20°C.

	==Labelling==		==Labelling==

Torsten Waldminghaus: /* General */

2012-06-27T12:04:25Z

General

←Older revision		Revision as of 12:04, 27 June 2012
Line 3:		Line 3:
	==General==		==General==

-	The strain collection consists of all strains which are constructed in the group or that we get from somewhere. All strains are stored as [[glycerol culture\|glycerol cultures]] in two copies, one at -80 and one at -20°C.	+	The strain collection consists of all strains which are constructed in the group or that we get from somewhere. All strains are stored as [[Waldminghau:Back Door/glycerol culture\|glycerol cultures]] in two copies, one at -80 and one at -20°C.

	==Labelling==		==Labelling==

Torsten Waldminghaus: /* Strain list */

2012-06-27T11:48:03Z

Strain list

←Older revision		Revision as of 11:48, 27 June 2012
Line 22:		Line 22:
	==Strain list==		==Strain list==

-	As much information as possible should be stored in the [[Waldminghaus:Back Door/Strains\|strain list]]. The list and the collection should always be consistent and updated!	+	As much information as possible should be stored in the [[Waldminghaus:Back Door/Strains\|strain list]].''' The list and the collection should always be consistent and updated!'''

Torsten Waldminghaus: /* Labelling */

2012-06-27T11:47:06Z

Labelling

←Older revision		Revision as of 11:47, 27 June 2012
Line 7:		Line 7:
	==Labelling==		==Labelling==

-	All strains are stored in boxes. Each box has a one-letter-label (A, B, C ...). When you start to work in the group you get one ~~box~~ to store your strains. In case you filled your box you get a new one.	+	All strains are stored in boxes. Each box has a one-letter-label (A, B, C ...). When you start to work in the group you get one of these boxes to store your strains. In case you filled your box you get a new one.

	Each tube that is put into the collection needs the following information on the tube:		Each tube that is put into the collection needs the following information on the tube:

Torsten Waldminghaus: /* Tube labelling */

2012-06-27T11:45:42Z

Tube labelling

←Older revision		Revision as of 11:45, 27 June 2012
Line 5:		Line 5:
	The strain collection consists of all strains which are constructed in the group or that we get from somewhere. All strains are stored as [[glycerol culture\|glycerol cultures]] in two copies, one at -80 and one at -20°C.		The strain collection consists of all strains which are constructed in the group or that we get from somewhere. All strains are stored as [[glycerol culture\|glycerol cultures]] in two copies, one at -80 and one at -20°C.

-	==~~Tube labelling~~==	+	==Labelling==
		+
		+	All strains are stored in boxes. Each box has a one-letter-label (A, B, C ...). When you start to work in the group you get one box to store your strains. In case you filled your box you get a new one.

	Each tube that is put into the collection needs the following information on the tube:		Each tube that is put into the collection needs the following information on the tube:

Torsten Waldminghaus at 11:37, 27 June 2012

2012-06-27T11:37:34Z

←Older revision		Revision as of 11:37, 27 June 2012
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	Each tube that is put into the collection needs the following information on the tube:		Each tube that is put into the collection needs the following information on the tube:
		+
	On the cap:		On the cap:
-	* Systematic name (A1-100, B1-100, C1-100,...)	+	* '''Systematic name''' - A1-100, B1-100, C1-100,...
	On the side:		On the side:
-	*	+	* '''Given name''' - If you constructed the strain this would be your initials plus a number starting at 1. If you get the strain from somewhere it probably has a name already.
		+	* '''Antibiotic resistence''' - For example amp, kan, cam, tet,...
		+	* '''Original parental strain''' - For example DH5α, MG1655, N16961,...
		+	* '''Plasmid''' - If it is a plasmid constructed in our lab the name should be according to our [[Waldminghaus:Back Door/Plasmid \|plasmid list]].
		+	* '''Relevant Genotype''' - For example pyrB::Tn5, proAB::GATC-cluster, ΔseqA,...
		+
		+	==Strain list==
		+
		+	As much information as possible should be stored in the [[Waldminghaus:Back Door/Strains\|strain list]]. The list and the collection should always be consistent and updated!

Torsten Waldminghaus: New page: {{Template:Waldminghaus}} ==General== The strain collection consists of all strains which are constructed in the group or that we get from somewhere. All strains are stored as [[glycerol...

2012-06-27T11:18:27Z

New page: {{Template:Waldminghaus}} ==General== The strain collection consists of all strains which are constructed in the group or that we get from somewhere. All strains are stored as [[glycerol...

New page

{{Template:Waldminghaus}}

==General==

The strain collection consists of all strains which are constructed in the group or that we get from somewhere. All strains are stored as [[glycerol culture|glycerol cultures]] in two copies, one at -80 and one at -20°C.

==Tube labelling==

Each tube that is put into the collection needs the following information on the tube:
On the cap:
* Systematic name (A1-100, B1-100, C1-100,...)
On the side:
*