WangLab:Coat Glass Bottom Dish with Fibronectin: Difference between revisions
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=== Follow this protocol if the transfected cells are to be passed on fibronectin-coated dish one day before imaging.=== | === Follow this protocol if the transfected cells are to be passed on fibronectin-coated dish one day before imaging.=== | ||
* Commercial fibronectin (bFn) has a concentration of 1mg/ml. They need to be diluted | * Commercial fibronectin (bFn) has a concentration of 1mg/ml. They need to be diluted 25 times with water to 20ug/ml. | ||
* Put uncovered dish with fibronectin ( | * Put uncovered dish with fibronectin (200ul/dish) in incubator overnight. Evenly spread the liquid in the well using pipette tip. | ||
* During passing the cells (right after centrifugation step), pipette out the excess liquid. | * During passing the cells (right after centrifugation step), pipette out the excess liquid. | ||
* Pass the cells to the fibronectin covered dishes. | * Pass the cells to the fibronectin covered dishes. | ||
NOTE: The above protocol is for MEF cells. | NOTE: The above protocol is for MEF cells. | ||
Revision as of 08:54, 26 May 2008
Follow this protocol if the transfected cells are to be passed on fibronectin-coated dish one day before imaging.
- Commercial fibronectin (bFn) has a concentration of 1mg/ml. They need to be diluted 25 times with water to 20ug/ml.
- Put uncovered dish with fibronectin (200ul/dish) in incubator overnight. Evenly spread the liquid in the well using pipette tip.
- During passing the cells (right after centrifugation step), pipette out the excess liquid.
- Pass the cells to the fibronectin covered dishes.
NOTE: The above protocol is for MEF cells.
