WangLab:HEPES buffer: Difference between revisions
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===HEPES=== | |||
*4-(2-Hydroxyethyl)-1-piperazineethanesuffonic acid | |||
*C8H18N2O4S | |||
*Molecular weight = 238.30 | |||
===HEPES buffer=== | |||
HEPES buffer | *20mM HEPES buffer, PH=7.3 | ||
*x/(238.30*0.1)*10^3 = 20 | |||
x | *x = 0.4766g | ||
*100ml H2O | |||
*1mol/L NaOH | |||
*x’/(40*0.1) = 1.0 | |||
x’ | *x’ = 4g | ||
*2g : 50ml H2O, 1g, 25ml H2O | |||
2g : 50ml H2O, 1g, 25ml H2O | |||
Latest revision as of 11:26, 20 June 2007
HEPES
- 4-(2-Hydroxyethyl)-1-piperazineethanesuffonic acid
- C8H18N2O4S
- Molecular weight = 238.30
HEPES buffer
- 20mM HEPES buffer, PH=7.3
- x/(238.30*0.1)*10^3 = 20
- x = 0.4766g
- 100ml H2O
- 1mol/L NaOH
- x’/(40*0.1) = 1.0
- x’ = 4g
- 2g : 50ml H2O, 1g, 25ml H2O
