WangLab:Magnetite Mcls: Difference between revisions
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*Bath Sonicator | *Bath Sonicator | ||
*Vortexor | *Vortexor | ||
*Lipid Mix | *Lipid Mix [1] | ||
#N-(a-trimethylammonioacetyl)-didodecyl-D-glutamate chloride (TMAG) | #N-(a-trimethylammonioacetyl)-didodecyl-D-glutamate chloride (TMAG) | ||
#dilauroylphosphatidyl-choline (DLPC) | #dilauroylphosphatidyl-choline (DLPC) | ||
Revision as of 12:41, 12 July 2007
Magnetite MCL Preparation
Materials
- Chloroform
- Bath Sonicator
- Vortexor
- Lipid Mix [1]
- N-(a-trimethylammonioacetyl)-didodecyl-D-glutamate chloride (TMAG)
- dilauroylphosphatidyl-choline (DLPC)
- dioleoylphosphatidylethanolamine-N-[3-(2-pyridyldithio)-propionate] (PDP-DOPE)
- Lipid marker (?)
- 10 nm magnetite nano-particles
- Specific RDG peptides
- Round bottom flask
Procedure
- Dissolve the three lipids (in a 1:2:2 molar ratio) into chloroform in the round bottom flask.
- Allow the chloroform to evaporate (~30 mins). Lipid film should coat the bottom of the flask.
- Suspend the magnetite nano-particles in water.
- Hydrate dried lipid layer on the bottom of the flask with nano-particle suspended water.
- Vortex the lipid/water/nano-particle solution for a while.
- Sonicate solution for 30 mins at 28 W in a bath sonicator to reduce to single laminar liposomes.
Links
- [1] Ito 2005 RDG peptide.pdf Outlines procedeure for production of RGD MCLs.
