WangLab:Protocols: Difference between revisions

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[[WangLab:Home | <font face="trebuchet ms" style="color:#ffffff"> '''Home''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[WangLab:Home | <font face="trebuchet ms" style="color:#ffffff"> '''Home''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[WangLab:Protocols | <font face="trebuchet ms" style="color:#ffffff"> '''Protocols''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[WangLab:Protocols | <font face="trebuchet ms" style="color:#ffffff"> '''Protocols''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[WangLab:Labmeeting | <font face="trebuchet ms" style="color:#ffffff"> '''Labmeeting''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[http://docs.google.com/Doc?id=dfbvms4w_15fn347khm <font face="trebuchet ms" style="color:#ffffff"> '''Labmeeting''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[WangLab:Order | <font face="trebuchet ms" style="color:#ffffff"> '''Order''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[WangLab:Order | <font face="trebuchet ms" style="color:#ffffff"> '''Order''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[WangLab:Biotin Avdin Blocking | Biotin Avdin Blocking]]
[[WangLab:Biotin Avdin Blocking | Biotin Avdin Blocking]]
[[WangLab:Cell Culture Protocols | Cell Culture Protocols]]
[http://docs.google.com/Doc?docid=dfbvms4w_3fzrjgzf7&hl=en Cell Imaging General Protocol (MEF)]
[[WangLab:Clustering of Epherin | Clustering of Epherin]]


[[WangLab:Coat Beads with Fibronectin | Coat Beads with Fibronectin]]
[[WangLab:Coat Beads with Fibronectin | Coat Beads with Fibronectin]]
[[WangLab:Coupling Protein Carboxyl Beads | Coupling Protein Carboxyl Beads]]
[[WangLab:Covalently Coupling Beads | Covalently Coupling Beads]]


[[WangLab:c-Src Mutations | c-Src Mutations]]
[[WangLab:c-Src Mutations | c-Src Mutations]]
[[WangLab:EGF Experiment | EGF Experiment]]


[[WangLab:Immunoprecipitation and Immunoblotting | Immunoprecipitation and Immunoblotting]]
[[WangLab:Immunoprecipitation and Immunoblotting | Immunoprecipitation and Immunoblotting]]
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[[WangLab:Immunostaining General | Immunostaining General]]
[[WangLab:Immunostaining General | Immunostaining General]]


[[WangLab:Coupling Protein Carboxyl Beads | Coupling Protein Carboxyl Beads]]
[[WangLab:Immunostaining Biotin | Immunostaining Biotin]]
 
[[WangLab:Cell Culture Protocols | Cell Culture Protocols]]
 
[[WangLab:Clustering of Epherin | Clustering of Epherin]]
 
[[WangLab:Covalently Coupling Beads | Covalently Coupling Beads]]
 
[[WangLab:EGF Experiment | EGF Experiment]]


[[WangLab:Immunostaining Biotin | Immunostaining Biotin]]
[[WangLab:Transfection | Lipofectimine 2000]]


[[WangLab:PH Domain GFPs | PH Domain GFPs]]
[[WangLab:PH Domain GFPs | PH Domain GFPs]]
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[[WangLab:Purify Protein Invitro Kinase Assay | Purify Protein Invitro Kinase Assay]]
[[WangLab:Purify Protein Invitro Kinase Assay | Purify Protein Invitro Kinase Assay]]
[[WangLab:RGD Peptide Inhibitions | RGD Peptide Inhibitions]]


[[WangLab:Ras and Src Treatment Protocol | Ras and Src Treatment Protocol]]
[[WangLab:Ras and Src Treatment Protocol | Ras and Src Treatment Protocol]]
[[WangLab:RGD Peptide Inhibitions | RGD Peptide Inhibitions]]


[[WangLab:Simple Purification of Adenovirus | Simple Purification of Adenovirus]]
[[WangLab:Simple Purification of Adenovirus | Simple Purification of Adenovirus]]
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[[WangLab:Transformation Procedure | Transformation Procedure]]
[[WangLab:Transformation Procedure | Transformation Procedure]]


[[WangLab:Lipofectimine 2000 | Lipofectimine 2000]]
 
 


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[[WangLab:Cell Culture Mediums | Cell Culture Mediums]]
[[WangLab:Cell Culture Mediums | Cell Culture Mediums]]
[[WangLab:Coat Glass Bottom Dish with Fibronectin | Coat Glass Bottom Dish with Fibronectin]]


[[WangLab:HEPES buffer | HEPES buffer ]]
[[WangLab:HEPES buffer | HEPES buffer ]]
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[[WangLab:Deletion and Addition Inserts | Deletion and Addition Inserts]]
[[WangLab:Deletion and Addition Inserts | Deletion and Addition Inserts]]


[[WangLab:Deletion or Insertion of Amino Acids w/o the Involvement of RE sites | Deletion or Insertion of Amino Acids w/o the Involvement of RE sites]]  
[[WangLab:Deletion or Insertion of Amino Acids w/o the Involvement of RE sites | Deletion or Insertion of Amino Acids w/o the Involvement of RE sites]]
 
[[WangLab:DNA Amplification | DNA Amplification (Mini prep)]]
 
[[WangLab:DNA Amplification (Maxi prep) | DNA Amplification (Maxi prep)]]


[[WangLab:DNA Digestion | DNA Digestion]]
[[WangLab:DNA Digestion | DNA Digestion]]
[[WangLab:Electro-competent | Electro-competent]]


[[WangLabs:FRET Related Techniques | FRET Related Techniques]]
[[WangLabs:FRET Related Techniques | FRET Related Techniques]]
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[[WangLab:Ligation Samples | Ligation Samples ]]
[[WangLab:Ligation Samples | Ligation Samples ]]
[http://docs.google.com/Doc?docid=dfbvms4w_5ctsj7ggp&hl=en Maxiprep procedure]
[[WangLab:Measure DNA Concentration | Measure DNA Concentration ]]


[[WangLab:Membrane Binding Domain Review | Membrane Binding Domain Review ]]
[[WangLab:Membrane Binding Domain Review | Membrane Binding Domain Review ]]
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[[WangLab:PCR Reaction Protocol | PCR Reaction Protocol]]
[[WangLab:PCR Reaction Protocol | PCR Reaction Protocol]]


[http://www.openwetware.org/images/f/ff/Primer_design.doc Primer Design]
[[WangLab:Primer Design | Primer Design]]


[[WangLab:Primer Design | Primer Design]]
[[WangLab:Stardard PCR | Standard PCR]]


[http://www.openwetware.org/images/6/6f/Standard_PCR.doc Standard PCR]
[http://docs.google.com/View?id=dfbvms4w_40cx7xv8fb Colony PCR]


[[WangLab|Stardard PCR | Standard PCR]]
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==Nanotechnology==
==Nanotechnology==
{| cellspacing="2"
|- valign="top"
|width="50%" class="MainPageBG" style="border: 1px solid #00007B; color: #000; background-color: white"|
<div style="padding: .4em .9em .9em">
<b>Protocols</b>
[[WangLab:Coupling Carboxyl Beads to EGF or Fibronectin| Protocols for Coupling Carboxyl Beads to EGF or Fibronectin]]
[[WangLab:PDMS Training | PDMS Training]]


[[WangLab:Silicon Master | Standard Protocol to Fabricate Silicon Master]]
[[WangLab:Silicon Master | Standard Protocol to Fabricate Silicon Master]]


[[WangLab:Coupling Carboxyl Beads to EGF or Fibronectin| Protocols for Coupling Carboxyl Beads to EGF or Fibronectin]]
</div>
 
|width="50%" class="MainPageBG" style="border: 1px solid #00007B; color: #000; background-color: white"|
<div style="padding: .4em .9em .9em">
<b>Materials</b>
 
</div>
 
|}


==Optics and Bio-Photonics==
==Optics and Bio-Photonics==
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[[WangLab:Alignment of Microscopy| Alignment of Microscopy]]
[[WangLab:Alignment of Microscopy| Alignment of Microscopy]]
[[WangLab:Lab Microscope Parameters| Zeiss Microscope Parameters]]
[http://docs.google.com/View?id=dfbvms4w_50jn3r9qjw Nikon Microscope Parameters]
[http://docs.google.com/View?id=dfbvms4w_51g8knznfc Starting Protocol for Nikon Microscope]


[[WangLab:Conversions| Conversions]]
[[WangLab:Conversions| Conversions]]
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[[WangLab:DIC Microscope Setting| DIC Microscope Setting]]
[[WangLab:DIC Microscope Setting| DIC Microscope Setting]]
[[WangLab:FRAP Experiment Protocol| FRAP Experiment Protocol]]


[[WangLab:Rules to Follow for Microscopy| Rules to Follow for Microscopy]]
[[WangLab:Rules to Follow for Microscopy| Rules to Follow for Microscopy]]
[[WangLab:FRAP Experiment Protocol| FRAP Experiment Protocol]]


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[[Category:WangLab_Page]]
[[Category:WangLab_Page]]
{| cellspacing="2"
|- valign="top"
|width="50%" class="MainPageBG" style="border: 1px solid #00007B; color: #000; background-color: white"|
<div style="padding: .4em .9em .9em">
<b>Protocols</b>
[[WangLab:operation of vacuum oven| Operation of Vacuum Oven]]
</div>
|width="50%" class="MainPageBG" style="border: 1px solid #00007B; color: #000; background-color: white"|
<div style="padding: .4em .9em .9em">
<b>Materials</b>
</div>
|}

Latest revision as of 11:30, 8 February 2010

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