WangLab:Stardard PCR
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Standard PCR
| Mg(1.5mM) | Mg(2.5mM) | Mg(1.5mM) | Mg(2.5mM) | |
| 1.10x buf (M190G, promega): | 5 | 5 | 5 | 5 |
| 2.MgCl2 | 3 | 5 | 3 | 5 |
| 3.DNA (miniprep): | 1 | 1 | 1(1/5) | 1(1/5) |
| 4.primer1 (20 uM): | 2.5 | 2.5 | 2.5 | 2.5 |
| 5.primre 2 (20 uM): | 2.5 | 2.5 | 2.5 | 2.5 |
| 6.*dNTP: | 1 | 1 | 1 | 1 |
| 7.*Tag: | 0.5 | 0.5 | 0.5 | 0.5 |
| 8.H2O: | 34.5 | 32.5 | 34.5 | 32.5 |
- Run program 10, all are good.
- Group 4 gives the best results.
