Week 9 Individual Journal William P Fuchs: Difference between revisions

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===Methods===
===Methods===
#Convert a nucleic acid sequence to a protein
#Convert a nucleic acid sequence to a protein
#*How do you know which of the six frames is the correct reading frame (without looking up the answer)?
*How do you know which of the six frames is the correct reading frame (without looking up the answer)?
**3-5' frame 3 made the most sense because it had a feasible amount of protein between the start and stop codons and had those stop/start codons.
**5'3' Frame 1 was the correct sequence because there was no stop codon in the reading frame.  
#Check the derived sequence with the actual data set to test the accuracy of the read.
*Check the derived sequence with the actual data set to test the accuracy of the read.
 
#Investigate gp120 further and find entries for that protein
*If you search on the keywords "HIV" and "gp120", in the main UniProt search field, how many results do you get?
**I got  206,278 hits
*Use the accession number "P04578" to narrow the search
===Results/Discussion===
===Results/Discussion===


===Data and Files===
===Data and Files===
[[Media:CheckSequenceSubject10V1C4.png]]


==Acknowledgements==
==Acknowledgements==

Revision as of 16:20, 25 October 2016

HIV Amino Acid Sequence Project

Question

Prediction

Target Clones and Visits

Rationale for Selections

In Class Activity

Purpose

To discover and implement various amino acid tools for the analysis and reconstruction of the protein structures of HIV.

Methods

  1. Convert a nucleic acid sequence to a protein
  • How do you know which of the six frames is the correct reading frame (without looking up the answer)?
    • 5'3' Frame 1 was the correct sequence because there was no stop codon in the reading frame.
  • Check the derived sequence with the actual data set to test the accuracy of the read.
  1. Investigate gp120 further and find entries for that protein
  • If you search on the keywords "HIV" and "gp120", in the main UniProt search field, how many results do you get?
    • I got 206,278 hits
  • Use the accession number "P04578" to narrow the search

Results/Discussion

Data and Files

Media:CheckSequenceSubject10V1C4.png

Acknowledgements

I worked with Matt Oki in developing the scope and direction of the amino acid project as well as developing the question, prediction and subject selection sections. While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source.William P Fuchs 18:18, 25 October 2016 (EDT)

References

Externals

Individual Journals

Class Journals

Assignments

Useful Links

  1. Bio Class Page
  2. BIOL368/F16:People
  3. Will Fuchs
  4. Link to LMU: http://www.lmu.edu/