Weiss Lab:TrafficPaper: Difference between revisions
(New page: Additional data for Dynamic Partitioning of a GPI-Anchored Protein in Glycosphingolipid-Rich Microdomains Imaged by Single-Quantum Dot Tracking Fabien Pinaud1,3, Xavier Michalet1,3, Go...) |
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Additional data for | Additional data for | ||
Dynamic Partitioning of a GPI-Anchored Protein in Glycosphingolipid-Rich | '''Dynamic Partitioning of a GPI-Anchored Protein in Glycosphingolipid-Rich Microdomains Imaged by Single-Quantum Dot Tracking''' | ||
Microdomains Imaged by Single-Quantum Dot Tracking | |||
Fabien | Fabien Pinaud[1,3], Xavier Michalet[1,3], Gopal Iyer[1], Emmanuel Margeat[1], Hsiao-Ping Moore[2], Shimon Weiss[1,3]. | ||
[1]Department of Chemistry & Biochemistry, University of California, Los Angeles, CA, USA. | |||
[2]Department of Molecular & Cell Biology, University of California, Berkeley, CA, USA. | |||
[3]Corresponding authors: | |||
fabien.pinaud@lkb.ens.fr; michalet@chem.ucla.edu and sweiss@chem.ucla.edu | [mailto:fabien.pinaud@lkb.ens.fr fabien.pinaud@lkb.ens.fr]; [mailto:michalet@chem.ucla.edu michalet@chem.ucla.edu] and [mailto:sweiss@chem.ucla.edu sweiss@chem.ucla.edu] | ||
Published in Traffic | '''Published in Traffic''' | ||
Note: The following additional data are part of the supplementary information that were submitted and reviewed for publication of the manuscript in Traffic. Unfortunately, because of size constrains, these additional data could not be posted together with the rest of the supplementary information on Traffic website. | Note: The following additional data are part of the supplementary information that were submitted and reviewed for publication of the manuscript in Traffic. Unfortunately, because of size constrains, these additional data could not be posted together with the rest of the supplementary information on Traffic website. | ||
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These data include: | These data include: | ||
Single-molecule dual-color total internal reflection fluorescence (TIRF) microscopy setups | |||
Crosslinking of Av-GPI induces patches in the absence of CTxB | |||
Comparison between single-trajectory PDSD analysis and global PDSD analysis | |||
Distribution of Av-GPI diffusion coefficients in untreated HeLa cells at 37°C | |||
Visualization of slow/immobile molecules using mean intensity projection images | |||
Immunostaining of endogenous caveolin-1 and colocalization with caveolin-1-EGFP in two HeLa cell lines | |||
Quantification of Av-GPI membrane expression in Av-GPI and Av-GPI/Cav1-EGFP expressing HeLa cells | |||
Diffusion coefficients and confinement sizes of Av-GPI for various treatments of HeLa cells at room temperature | |||
Revision as of 12:12, 16 February 2009
Additional data for
Dynamic Partitioning of a GPI-Anchored Protein in Glycosphingolipid-Rich Microdomains Imaged by Single-Quantum Dot Tracking
Fabien Pinaud[1,3], Xavier Michalet[1,3], Gopal Iyer[1], Emmanuel Margeat[1], Hsiao-Ping Moore[2], Shimon Weiss[1,3].
[1]Department of Chemistry & Biochemistry, University of California, Los Angeles, CA, USA.
[2]Department of Molecular & Cell Biology, University of California, Berkeley, CA, USA.
[3]Corresponding authors: fabien.pinaud@lkb.ens.fr; michalet@chem.ucla.edu and sweiss@chem.ucla.edu
Published in Traffic
Note: The following additional data are part of the supplementary information that were submitted and reviewed for publication of the manuscript in Traffic. Unfortunately, because of size constrains, these additional data could not be posted together with the rest of the supplementary information on Traffic website.
These data include:
Single-molecule dual-color total internal reflection fluorescence (TIRF) microscopy setups
Crosslinking of Av-GPI induces patches in the absence of CTxB
Comparison between single-trajectory PDSD analysis and global PDSD analysis
Distribution of Av-GPI diffusion coefficients in untreated HeLa cells at 37°C
Visualization of slow/immobile molecules using mean intensity projection images
Immunostaining of endogenous caveolin-1 and colocalization with caveolin-1-EGFP in two HeLa cell lines
Quantification of Av-GPI membrane expression in Av-GPI and Av-GPI/Cav1-EGFP expressing HeLa cells
Diffusion coefficients and confinement sizes of Av-GPI for various treatments of HeLa cells at room temperature
